The Differences Of FATP1/FATP4 Gene Expression Of Different Tissues And Developmental Stages In Goose

At present, several candidate proteins have been proposed to be involved in the uptake process of LCFAs including fatty acid transport proteins (FATPs),fatty acid binding protein (FABPs), long-chain fatty acyl-CoA synthetases (ACSL) and fatty acid translocase (FAT/CD36).Although many of the above-mentioned proteins have important role in the proceeding of LCFAs uptake and metabolism, recent in vivo studies have particularly highlighted the contribution of FATPs to lipid metabolism and fatty acid-associated disorders. So,the studies about FATP are being focused in today because of FATP special character and biological function.In the studies, we adopted East White Geese and Landes Geese as materials. We used the real-time PCR (SYBR green I) method to test the expression quantity of FATP1 and FATP4 gene in different tissues and the expression quantity at different growth points.The results showed that:the expression quantity of FATPl gene in breast muscle reached the highest expression in 35 day,and reached the lowest expression in 49 day in the two breeds;the expression quantity of FATP1 gene in leg muscle reached the highest expression in Zhedong White Geese in 63 day,and reached the highest expression in Landes Geese in 35 day; the expression quantity of FATP1 gene in Sebum reached the highest expression in 21 day in Zhedong White Geese,and reached the highest expression in Landes Geese in 14 day,and after sharply;the expression quantity of FATP1 gene in abdominal fat reached the highest expression in Zhedong White Geese in 42 day,and reached the highest expression in Landes Geese in 35 day; the expression quantity of FATP1 gene in liver reached the highest expression respectively in Zhedong White Geese and Landes Geese in 1 and 28 day; the expression quantity of FATP1 gene in heart reached the highest expression in Zhedong White Geese in 56 day,and reached the highest expression in Landes Geese in 63 day.The results also show that the expression quantity of FATP4 gene in breast muscle reached the highest expression respectively in Zhedong White Geese and Landes Geese in 63 and 35 day; the expression quantity of FATP4 gene in leg muscle reached the highest expression in 56 day in the two breeds; the expression quantity of FATP4 gene in Sebum reached the highest expression in 21 day in Zhedong White Geese,and reached the highest expression in Landes Geese in 14 day,and after sharply; the expression quantity of FATP4 gene in abdominal fat reached the highest expression in Zhedong White Geese in 35 day,and the expression of Landes Geese except 14 day the other times points is relatively stable quantity of expression; the expression quantity of FATP4 gene in liver reached the highest expression respectively in Zhedong White Geese and Landes Geese in 56 and 14day; the expression quantity of FATP4 gene in heart reached the highest expression in Zhedong White Geese in 56 day,and reached the highest expression in Landes Geese in 63 day. the expression quantity of FATP1 and FATP4 had many expressions between the two breeds and at different growth points and tissues.Moreover,the expression of abundance have difference. The expression of advantage tissue is leg muscle between the two breeds and the expression is significantly higher than other organizations (P<0.05).The results showed that the expression quantity of FATP1 had significant difference between the two breeds in leg muscle and breast muscle, the difference of expression is significantly(P<0.05),and the other tissues had no significant differences(P>0.05);the expression quantity of FATPA had no significant difference between the two breeds(P>0.05). The expression of FATP1 gene is higher than FATP4 gene.In the studies,we found that the expression quantity of FATP1 gene had small trace expression in intestine.This is belong to first discovered in Landes geese and Zhedong White geese. Thus, the expression of FATP4 gene in the small intestine is not the only members in FATPs family.