Spleen-Asthenia Syndrome With The Function Of Hypothalamus-Pituitary-Thyroid-Thymus Axis

The spleen in TCM is closely linked to the neuroendocrinimmunology (NET) net in Modem Medicine, spleen-asthenia will lead to the impairment of the immunity function .The study on "Essense of Spleen-.Asthenia"has dealt with it. in the present experiment , the subject - "relationship of Spleen- Asthenia Syndrome with the function of hypothalamus- pituitary-thyroid- thymus axis", was studied . Their correlation was studied by using immunoradiometric assay , radioimmunoassay , HPLC-EC to observe a series of parameters. The parameters showed the changes of the levels of serum T3 T4, FT3. FT4, y T3 , the contents of nuclear T3 receptors in hypothalamus cells & thymus T lymphocytes , the contents of TRH hypothalamic, TSH pituitary , TRH, TSH serum levels ,the contents of L-EK brain tissues , and serum levels , the levels of NE in frontal cortex , and the expression of IL-2 levels of thymus T lymphocyte . Results as follow: 1. Changes of the levels of rat serum T3, T4, FT3, Fl4, y 13 In SPL and SPO , T3 ,T4 , FT3 ,FT4 rat serum levels, were lower than those in CON and TRE (P,zO.O5 or P O.O 1); in these groups, y -T3 serum levels had no significant difference. The parameters in CON and TRE had no significant difference(P>O.05). 2. Changes of the contents of nuclear 13 receptors in hypothalamus cells & thymus I lymphocytes in SPL and SPO , the contents of nuclear 13 receptors in hypothalamus cells and in thytnus T lymphocytes were remarkably lower than those in CON and IRE ( PO.05 ). 3. Changes of the contents of TRH hypothalamic, TSH pituitary, and TRill, TSH rat serum levels In SPL and SPO ,the contents of TRJ-I hypothalamic, TSR pituitary levels were remarkably lower than those in CON and IRE (P?O.O 1); In SPL and SPO , the contents of IRK, TSR serum levels were remarkably higher than those in CON and TRE (PO.05). 4. Changes of the contents of in L-EK brain tissues , and serum levels In SPL and SPO , the contents of L-EK serum levels were remarkably lower than those in CON and IRE (PO.05). 5. Changes of the contents of the levels of NE in frontal cortex , and IL-2 levels of thymus T lymphocyte In SPL and SPO , the levels of NE in frontal cortex and the levels of IL-2 of thymus T lymphocyte were remarkably lower than those in CON and TRE ( PO.05). Conclusion: 1 .Impaired hypothalamic- pituitary-thyroid regulation function may be attributed to progression of spleen-asthenia. 1 .1 The decline of the expression of IL-2 levels of thymus I lymphocyte may lead to the impairment of immunity. 1.2 The decline of the levels of serum T3, 14, and the contents of nuclear T3 receptors in thymus I lymphocytes , may lead to the impairment of immunity. 1.3 The decline of the levels of serum 13 T4, and the IRK hypothalamus levels makes TSH pituitary levels lower, and the function of hypothalamus- pituitary- thyroid- thymus axis is weaker. 1.4 The decline of the levels of NE in frontal cortex, and the increase of L-EK brain tissues are attributed to the decline of IRK hypothalamus levels & TSR pituitary level...