Studies On The Chemical Constituent And Antiviral Active Sites Of Isatis Indigotica Fort.

Abstract Abstract 10 natural compounds were seperated from the antiviral site of Isatis indigotica Fort. by solvent and chromatography. 7 of them were indentified by spectroscopic methods, physiochemical properties and literature data. Among them, there was one novel compound and six known compounds. The novel compound was 3-.(4-O- ~ -D-glucopyranosyl- ci -hydroxy- 3 -methoxy-benzyl)-4-(4-hydroxy-3 -methoxy-benzyl)tetrahydrofuran (Indigoticoside A, 1-2). The know compounds were respectively: (+)-isolariciresinol (I-i), Epigoitrin (I-i 0), 5-hydroxymethyl-furadehyde (1-4), 5-hydroxymethyl -fttroic acid (1-5), n-butyl- ~ -D-fructopyronoside (1-6), sucrose (1-3). Among them, I-i, 1-4, 1-5, 1-6 were isolated from Isatis genus for the first time. In the course of structural determination, 2D-NIvIIR techniques, especially HMIB C spectrum, were successfiully applied to determine the position of the substitute groups and sugar in the structure of lignan glycoside. æ…” and ?C-signals of novel compound 1-2 were assigned by means of 2D-NMR spectra. The pharmacological test showed that n-butanol extract and 80% ethanol elusion fraction obtained from D,0, polymer adsorption column had strong antiviral effects in vitro.