| Backgrond and Objective:Promoting blood circulation by removing blood stasis is one of the important therapy by which the Traditional Chinese Medicine (TCM)treat the coronary heart disease. Many drugs of promoting blood circulation by removing blood stasis have been screened by dometic researchers recent years.Recent progresses have been made in the field of these drugs' action mechanism.The concept of protecting the function of vascular endothelium has been payed great attention to. Previous study showed that the drugs of promoting blood circulation by removing blood stasis are effective in treating myocardial ischemia and myocardial infarction. Qidan tongmai tablet is acompound preparation made of pure Chinese herbs which has the effect of supplementing qi, activiating blood circulation, relieving pain by removing obstruction in the channels .To investigate the Tablet's protection on the cardiac function of ischemic myocardium and it's effect on the gene expression level of NOS/ET ,which are the vascular endothelium-derived active substances,the following studies were carried out. Subjects and Methods(1)Duplicating rabbit model of acute myocardial ischemia by means of ligating left ventricular branch(LVB) of coronary artery, observing and measuring dynamic characteristics of cardiac mechanical function, indexes such as 眃p/dtmax.LVP, LVEDP> MAPs HR.(2) Removing the rabbit heart, making myocardium section preparation, the NOS and ET gene expressions in the ischemic myocardium were observed by in situ hybridization. The Main ResultsIt was found that 眃p/dtmax> LVP> MAP display a decling trend after coronary ligation while the index of LVEDP showed a rising trend after ligation .The +dp/dtmax in qidan tongmai tablet large dose group has decreased by 25.5% , 34.1% at 120 and 180 min after ligating LVB;which is different from the ischemia model group(P<0.05) at the same timepoint left ventricular systolic pressure(LVSP) in qidan tongmai tablet large dose group has decreased by 14%,23% after ligation two hours and three hours respectively which is much different from the ischemia model group (P<0.05) at the same time point.The small dose group of qidan tongmai tablet has decreased in LVSP by 21.2% which is not much different from the Model group.yet the decreased level is less than that of the model group.In the large dose group(QDTMT),MAP was decreased by 13.2%> 18.0%at 120mirK ISOmin respectively ,which is different from the model groupThe large dose group(QDTMT) was decreased in -dp/dtmax by 9.2%> 34% at 120min> ISOmin respectively, which is different from that of the model group(.P<0.05).In the same group,LVEDP was decreased by lll.l%(P<0.05 vs model) 150%CP<0.01 vs model).319.4%(P<0.05 vs model )at 60miiK 120miiK 1 SOmin respectively. The result of the in situ hybridization.(1)NOSmRNA: The possitive signals of the hybridization result are pale brown granulars. which distributed mainly in the cytoplasm. The ischemia model group showed light pale brown color and limited signals,which indicated that the transfer of NOSmRNA was greatly decreased.The positive signals in the qidan tongmai tablet large dose group are dence and of deep pale brown color The small dose group also showed more positive signals. than that of the model group.(2)ETmRNA: The possitive signals are pale brown granulars in all experimental group and distributed mainly in the cytoplasm .There were a great deal of possitive signals in the ischemic myocardium .which showed dence> deep pale brown granulars.In the Ditazem group,.the qidan tongmai groups the signals were decreased,and the large dose group of the tablet was esptcially notable by its greatly decreased signal. The Conclusions and Suggestions(1) Rabbit was an ideal experimental animal of duplicating myocardial ischemia model .(2) Qidan tongmai tablet improved the systolic and diastolic function of theischemic myocardium which indicated that this preparation have the effect of protecting ischemic myocardium.(3)The t...
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