Expression Of CD11a/LFA-1, CD54/ICAM-1 And Level Of Serum SICAM-1 In Acute Leukemia

Cell adhesion molecules(CAMs) play important roles in maintaining the contract between cells and are essential for inflammation ,immune reaction and migration of neoplastic cells. It has been known that CAMs involve not only in the proliferation and survival of leukemic cells ,but also in extramedullary invasion and metastasis of cells.This study was to investigate the frequency of CD 11 a, CD54 expression of bone marrow mononuclear cells and the level of serum soluble ICAM-l(sICAM-l) in acute leukemia (AL) and the correlations with CAMs and clinical feature.Method: The frequency of CDlla,CD54 expression of bone marrow samples from 20 acute lymphocytic leukemia(ALL), 27 acute myeloid leukemia(AML), 16 controls were measured by flow-cytometric analysis , at the same time the levels of serum sICAM-1 were also measured by ELISA.Results:The frequency of GDI la, CD54 expression in ALL group (41.67?10. 33, 35. 03 + 13. 09) and in AML group (48.97 ?12. 93, 26. 71 ?. 70) were both lower than the control(76.82?. 41, 70. 04?. 89) (P<0. 01, P<0. 01) .There were no significant difference between T-ALL group and B-ALL group, and no difference among subtypes of AML. The CDlla,CD54 expression of complete remission(CR) group of ALL and AML had no difference with the control, whereas the expression of unremission group were still lower than the control. In extramedullary invasion group the frequency was higher than that of no invasion group. The levels of serum sICAM-1 of two leukemia groups were both higher than the control. When patients reached CR, the levels of sICAM-1 fell to the normal level and the unremission group had high level of sICAM-1 than the remission group before chemotherapy. The level of serum sICAM-1 in leukemic patients was significant correlated with the frequency of CD54 expression .Conclusiorr.The frequency of CD 11 a, CD 54 expression in AL cells were lower than the control and related to the release of the leukemic cells from bone marrow and fell to the normal when the patient had reached CR afterchemotherapy. The CAMs expression also had relationship with extramedullaryinvasion of leukemic cells. The high level of serum sICAM-1 in leukemicpatients related to immune escape and tended to relate to prognosis. Moreover ,thelevel of sICAM-1 had significant correlation with frequency of CD54 expression,it appeared that serum sICAM-1 may derived from shedding of CD54 from cellsurface.