| Object The purpose of our study is to explore if there is the difference of effects on male reproductive system of iodine deficiency rat by the two kinds of iodate salt in different dosage.Methods In our study, firstly, Wistar male rat model of iodine deficiency was made successfully ; secondly, rats were randomly divided into five groups: LI, NO, NI, HO and HI. Rats were killed after 22 weeks, then weights of reproductive organs and accessorial sex glands, level of urinary iodine, antioxidant capacity and MDA content of testis and epididymis, concentration of serum testosterone (T) and morphological change of testis were investigated by using physical, chemical, radioimmunological and histological methods.Results 1 Weights of rat reproductive organs and accessorial sex glands in five groups demonstrated that absolute and relative weights of testis, epididymis, prostate and preputial gland in group LI were the lowest among the five groups. There was statistical difference between group LI and HO in absolute and relative weight of testis (p<0.05) -, compared with group NO, NI, HO and HI, absolute and relative weight of epididymis in group LI decreased significantly (p<0.05) ; absolute and relative weight of preputial gland in group LI decreased markedly compared with group NI, HO and HI (p<0.05). There was no striking difference in testis volume among the five groups (p>0.05). 2 The level of urinary iodine of group LI in 24 hours was nearly zero; the levelof urinary iodine of group NO in 24 hours was much higher than that of group NI (p<0.01); the level of urinary iodine of group HI in 24 hours was markly higher than that of group H0(p<0. 01); the level of urinary iodine of group HI and HO in 24 hours was nearly 100 times to that of group NI and NO. 3 There was no statistical difference in antioxidant capacity of rat testis and epididymis between group NO and NI (p>0. 05), and so did between group HO and HI. Compared with group NO , NI and LI , testis glutathione peroxidase (GPx) activity in group HO and HI strikingly increased (p<0. 01). There was no significant difference in testis superoxide dismutase (SOD) activity among the five groups (p>0.05). Compared with group NO and NI , testis malondialdehyde (MDA) content in group HO, HI and LI significantly increased (p<0.01), moreover, testis MDA content in group HO and HI was much higher than group LI (p<0.01). Epididymis GPx activity in group HO was strikingly higher than group NI and LI (p<0.05), and there was a tendency to increase and decrease in group HI and LI respectively. Similarly, there was no statistical difference in epididymis SOD activity among the five groups (p>0.05). Equally, epididymis MDA content in group HO, HI and LI was much higher than group NO and NI(p<0. 01), furthermore , epididymis MDA content in group HO and HI was significantly higher than group LI(p<0.01). 4 There was no statistical difference in the concentration of T among the five groups (p>0.05). 5 Histological examination indicated that the number of Leydig cell of rat testis in group HO and HI increased.Conclusions 1 Urinary iodine level of the five group rats paralleled with the iodine content in drinking water. 2 Long period of iodine deficiency could result in the weights of male rat reproductive organs and accessorial sex glands decreasing and lipid peroxidaciton damage to testis and epididymis, which might reduce rats capacity of fertility. 3 The normal dosage of KI03 and KI had better treatment on iodine deficiency rats and the dosage was not detrimental to male rat reproductive system; there were no different effects between KIOs and KI. 4 Excessive supplement of KIOs and KI to iodine deficiency rats could lead to worse peroxidation damage to testis and epididymis, which might reduce rats capacity of fertility. 5 Long period of iodine deficiency and supplement of normal or excessive KI03 and KI to iodine deficiency rats had no effect on the concentration of serum testosterone(T). 6 The histological examination found that Leydig cell of rat testis hype...
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