| Syphilis, caused by Treponema pallidum^P), is a chronically, systematically and sexually transmitted disease(STD) with complicated clinic exhibition. During the long and chronic course of disease, patient's apparent symptoms alternate with latency ones. The early stage syphilis (less than 2 years), due to its strong infectivity is the main source of infection. Nevertheless, the advanced stage syphilis (more than 2 years), though the tissue destroyed seriously, comparatively has a little of infectivity. The longer the course of the disease has, the weaker the infectivity is. Syphilis can destroy every tissue or organic on people's body and even lead to lameness. Up to now , the real mechanism of this disease has not be found. The general treatment to syphilis isrecommended by WHO in 1981 is to use penicillin as the first choice only vary in dosage and period of treatment according to different course of disease and state of illness.In recent years, along with the increasing incidences of domestic syphilis, more and more attention is paid to such disease. The study to this disease is mostly concentrated on the serum detection and antigen of Treponema pallidum for syphilis patient, few attention is paid to the immunity function of syphilis patient. However, in our clinical practice, disappearance destroyed skill and RPR seronegative differed from one patient to another under the same treatment, which made us consider and study whether or not that result was caused by the individual immunity difference. In recent years , though immunology progress greatly, the study to syphilis immunology has little advance. The immunity principle has not been cleared after patients receiving Treponema pallidum, but the close relationship between syphilis and immunity is clear. It is not difficulty to see from clinical that the course of disease is related to humoral immunity and cellular immunity no matter that is proved by either clinical expression or animal test. This dissertation, from the point of view of cellular immunity, studied the syphilis patient's immunity function and the relationship between the course of disease and treatment results so that it can be of much help to the more effective treatment and prognosis judgement.Materials and methods:With flow cytometry, using CD monoclonal antibodies labled by either fluorescein isothiocyanate (FITC) or phycolerythrin(PE), special feature of peripheral blood lymphocyte immunophenotypes (PBLI) was detected through pre-treatment and post-treatment of 46 cases of syphilitic patients and 20 cases of normal persons. Meanwhile, the same patients' IL-2 and sIL-2R serum level was detected by the ELISA method. After three month treatment, patients were divided into two groups according to their RPR result: group A, the seronegative; and group B, the seropositive. This report analyzed the difference of cellular immunity function between syphilis patients and normal group, and the one difference cellular immunity function among different courses of disease, even more the relationship between the different cellular immunity function and the different treatment results. Experiment data were statistically processed by computer with SPSS(10.0) software system, measure data were presented by x + s, analysis of variance(ANOVA)were used for multigroup materials and g-test were used to examine the discrepancy of multiple comparisons in multimeans , two sample means with Mest or t'-test to examine the notable discrepancy, pair Mest were used to examine the discrepancy of before and after treatment. Probability values less than 0.05 were considered statistically significant.Results:1 . Compared the cellular immunity function of syphilis patient before treatment with normal control persons', CD3+ cells, CD4+ cells, the ratio of CD4+/CD8+, NK(CD,6+, CD56+ )cells and the level of IL-2 in syphilic patients were significantly decreased ( P<0.01); but CD8+ cells and the level of sIL-2R significantly increased (P<0.01).2. The comparison cellular immunity function of syph...
|
PDF Full Text Request