The Expression Of VEGF, MVD And P16 In Cholangiocarcinoma And Their Clinical Significance

Objective: Vascular endothelial growth factor(VEGF) plays an important role in the angiogenesis of carcinoma. With selective function on receptors of VEGF, VEGF significantly stimulates the growth of Vascular endothelial cells in tumor and increases the permeation of tumor vasculature. Microvessel density( MVD )is an important index to reflect the angiogenesis of carcinoma. MVD not only relates to the supply of nutrition and oxygen in growth of tumors,but also indicates tumors permation and metastasis. Being a kind of tumor suppressor, p16 gene plays an important role in growth of many tumors by special inhibition on CDK4. Present research about the expression of VEGF, MVD and p16 in cholangiocarcinoma is not adequate. In the present study, we investigate the expression of VEGF, MVD and p!6 and corelation of them in cholangiocarcinoma.Method:The expression of VEGF, MVD and p16 were examined by SP immunohistochemical methods in surgical specimens of 40 cases with cholangiocarcinoma and 10 non tumorous cases. Then, we investigate the difference ofexpression of VEGF, MVD and p16 between cholangiocarcinoma group and control group. At the same time, we analyze relations among VEGF, MVD and p16.Results:The expression of VEGF, MVD and p16 are 77.5%,64.5+36.23 and 60% in cholangiocarcinoma group and 40%, 26.6+15.32 and 100% in control group. There are significantly different expressions between two groups in VEGF, MVD and p16. There are significant correlation between VEGF and MVD, and p16.Conclusions: Significant increases in expression of VEGF and MVD in cholangiocarcinoma group indicate that both VEGF and MVD play important roles in cholangiocarcinoma. Significant decrease in expression of p16 in cholangiocarcinoma group suggest that p16 may inhibit the growth of cholangiocarcinoma. Statistic analysis also indicates that significant increase in VEGF is responsible for significant increase in MVD and decrease of p16 may answer for the increase of VEGF. These results suggest that VEGF overexpression and p16underexpression are associated with intratumoral angiogenesis, growth and metastases.