Relationships Between Mad2 And P53 Expression In Colorectal Cancer And Their Clinical Significances

Objective: To study the expression of Mad2 gene and p53 gene and to evaluate their clinical significances in colorectal cancers. Methods: Inmmunohistochemistry method and Western blot were used to analyze the expression of spindle checkpoint gene Mad2 between colorectal cancers and their corresponding normal mucosa. Inmmunohistochemistry method were used to analyze the expression of p53 gene between colorectal cancers and their corresponding normal mucosa. Results: Mad2 was significantly overexpressed in colorectal cancers compared with their corresponding normal mucosa (P < 0.001),and it wasn't related to the differentiation of adenocarcinomas and the other clinical factors(P > 0.05). Gene p53 protein expression was not observed in normal mucosa. The rate of p53 positive expression in adenocarcinomas was 52.6% .There was significant difference between normal mucosa and adenocarcinomas(P < 0.001), and it wasn't related to the differentiation of adenocarcinomas and the other clinical factors (P > 0.05). The calculated ratio of Mad2 protein in cancer tissue(C) to that in their corresponding normal mucosa tissue(N) was higher than 2 more frequently in patients with lymph gland metastasis and C, D phase in Duke's division(P<0.05). Conclusion : Our results indicate that the mutation of p53 gene and defect of spindle checkpoint gene Mad2 are involved mainly in colorectal carcinogenesis and C/N>2 was associated with prognosis of colorectal cancer.