Effect Of Human Growth Hormone On Cell Cycle Kinetics In GRC—1 Cells Line In Vitro

Objectives f Renal cell carcinoma sti1l is an important disease threateninghuman life. TO StUdy a new method for the treatInent of renal cell carcinoma and explorethe effect of human groed hormone on cell cycle kinetics and aPoPtosis of human renalcarcinoma cell line (GRC-l cells). Methodsi GRC-1 cells were cultured fOr 24 hour inl640 with or edout Fluorouraci, but whh rhGH at a concentration of 50,l00,200ng/ndrespeCtive1y Cells undergoing aPoptosis and differentiation wee determined by flowcytometryGCap. Results f rhGH had significant effect on GRC-l cell cycle rate, percentG0-G, phases dropped (P<0.05), percent S phase increased (p<0.0l). addition QfFluorouraci to the culture medium could drop all rates of S phase. Addition of rhGH atdifferent concentration and Fluorourac to the culture medium could drop cell rates of Sphase deeply Conclusions; GRC-l cells might have exPression fOr the GH recePtor .Invitro rhGH ndght induce differentiation of cells GRC-l and promote cell cycle kineticsand Strengthen Fluorouraci suppressing on percent S phase.