Relationship Of BFGF,EGFR Expression With Angiogenesis And Metastasis In Lung Cancer And Blood Vessel In Vivo Imaging

Purpose The purpose of this research was to explore the relationship of basic fibroblast growth factor (bFGF) and epidermal growth factor receptor (EGFR) with angiogenesis and lymph node metastasis of non-small cell lung cancer (NSCLC), and to analyze the blood vessel imagings of lung cancer transplanted BALB/c mice in vivo.Methods The expression of bFGF, EGFR and CD34 was examined by immunohistochemical technique in 62 NSCLC and 10 benign lung lesions. In situ hybridization was used for further detecting of bFGF mRNA expression. In addition, the animal model of lung cancer transplanting BALB/c mice was established, and laser scanning confocal microscopy (LSCM) was used to scan continually the superficial vascular system of lung cancer in BALB/c mice to distinguish the difference between the experimental and the control groups in vivo.Result The level of bFGF, EGFR protein expression and the microvessel density (MVD) of tumor tissues in differentiated grading III were remarkably higher than in differentiated grading I~II, and remarkably higher in III-IV stage than in I~II stage, and significantly higher in lymph node metastasis group than in non-metastasis group (P<0.05). Furthermore, the MVD of NSCLC displaying bFGF-positive /EGFR-positive was higher than that of the inverse conditions (P<0.05). In addition, the result of laser scanning image showed that vascular system of the experimental tumors were thicker, and the density was higher than the controls (P<0.05).Conclusions It was suggested that overexpression of bFGF and EGFR were related to the differentiation, clinical stage, angiogenesis and lymph node metastasis of NSCLC. To detect the expression of bFGF, EGFR and count theMVD may present more reference and biological information in pathological diagnosis, evaluation of prognosis and clinical treatment of NSCLC. In addition, LSCM was valuable to check the spatial structural relationship of the lung carcinoma vascular system, and may provide a new method for further investigating the neoplastic angiogenesis in vivo.