Preparation And Characterization Of Liver-targeting Mitomycin C-polybutylcyanoacrylate Nanoparticles

Nanoparticles are small polymeric particles in the nanometer size range. Usually They are easily taken up by phagocytic cells and accumulate in the organs of the reticuloendothelial system (RES) such as the liver,the spleen and the lympth. As a drug carrier system,it has the ability of tumor targeting,changing the biodistribution of drugs,controlled or sustained drug release,reducing the toxicity and enhancing the efficacy of drugs.In this article,polybutylcyanoacrylate was used as the polymeric carriers,and the model drugs we chosed was mitomycin C. After serials of experiments,we found a new method to prepare mitomycin C-polybutylcyanoacrylate nanoparticles in neutral pH aqueous medium,which solved the problem that polybutylcyanoacrylate nanoparticles usually were reported be prepared in low pH aqueous medium,while mitomycin C was unstable in such conditions.Technical conditions were determined by uniform design. The morphology,size and size distribution,encorperation rate,drug loading,release characteristics in vitro,biodistribution of mitomycin C-polybutylcyanoacrylate nanoparticles in mice were studied. The results showed that the average size was 79.87+ 7.24nm,span=1.60,encorporation rate was 79.03%,drug loading was 6.58%,the release characteristics in vitro was in accord with logarithmic kinetics,76.29% of the mitomycin C were accumulated in liver at 30min after iv MMC-PBCA-NP. The study showed a possibility of enhancing efficacy and reducing toxicity of mitomycin C.