Studies On Anti-Myocardial Ischemia Effects And Mechanism Of Action Of Guanxin-Suhe Delayed-Release Capsule

The anti-myocardial ischemia effects and the mechanisms of Guanxin Suhe Delayed-Release Capsule(DRC) were preliminarily studied. Toxicity and the main pharmacokinetic parameters of DRC were also studied in this paper.DRC treatment prolonged the survival time of mice and the duration of electrocardiograph in trachea closed mice.DRC could obviously improve the ischemic electrocardiogram T-wave induced by Pituitrin (Pit). ST segment drifts on electrocardiogram in acute myocardial ischemia rats induced by Isoprotereno (Iso) were obviously opposed by administration of DRC. The myocardial infarction areas, releasement ofmyocardium enzyme aspartate aminotransferase (AST), lactic dehydrogenase(LDH), creatine phosphokinase (CPK) significantly increased in myocardialischemia dogs, DRC treatment prevented these effects. These results indicated that DRC had the protective effects on ischemia myocardium. In hemodynamics studies of coronary ligated dogs, the left ventricular systolic pressure (LVSP); ventricular pressure maximal change rate (眃p/dt); coronary blood flow (CBF);cardiac output (CO); myocardial blood flow (MBF) decreased after coronary ligated, while left ventricular end diastolic pressure (LVEDP) increased in dogs. These changes were prevented by administration of DRC, which showed that DRC could prevent the cardiac pump function from acute myocardial ischemia of coronary ligated dogs and increase blood supplement to myocardium. Moreover, DRC showed obvious anti-platelet aggregation and anti-thrombosis effects and significantly reduced thewet thrombus weight in rabbits. The hemorheology in the rat model of blood stasis could also be improved by treatment of DRC. In pharmacokinetic studies, the mean residence time (MRT) of DRC was 9.96 hours, while the relative bioavailability(Frel )was 121.82%.Summarizing all the experimental results, DRC showed anti-myocardial ischemia effects. The mechanism maybe involved in ameliorating hemodynamical indexes, increasing the superoxide dismutase (SOD) activity, inhibiting oxygen free radical(OFR) activity, stabilizing myocardial cell membrane, anti-plateletaggregation effects, increasing 6-Keto-PGF1 a/TXB2 rate and ameliorating hemorheology.