| Along with the rapid progress of the heart transplantation, the heart preservation solution should be improved. The University of Wisconsin solution(UWs) ,which has been reported to extend cold ischemic time in preservation of the liver, kidney, and pancreas, has been used widely in our country. But its value is limited in the heart transplantation because of its higher incidences of blood vessel complication, high viscidity, high price and the ability to preserve only in short time. So, in the based of Uws and Bretschneider's histidine-tryptophan-ketoglutarate (HTKs), we try to prepare a novel solution for prolonged heart preservation.Objective In order to improve protecting effects of heart, prolong the time ofpreservation and reduce postoperative complications, in this study we try to prepare a new effective, convenient solution which can preserve isolated heart forlong time. Methods Having analyzed the compenents of the UW and HTK solution, we pull off the hyperpolarized cardiac solution including following integrants: Sodium lactobionic acid 80mmol/L, KC1 10mmol/L, MgSO4· 7H2O 4mmol/L, CaCk 0.15mmol/L, Bicine 90mmol/L, Nicorandil 0.1mmol/L, Adenosine 5mmol/L, Glutamyl 20mmol/L, L-arginine 2mmol/L, Allopurinol Immol/L, Dextran-40 50g/L, pH 7.40±0.10, Osmolarity (mmol/L): 310-330. Male Sprague-Dawley (200-250g, n=24) rats are randomly divided into three groups, with eight in each, group 1 serves as K-H solution, group 2 as UWs, and group 3 as HCS. The isolated hearts were excised and immediately perfused in the Langendorff rat work mode for functional evaluation, then were immersed in 4℃ solution for 24h. In all hearts, left ventricular summit pressure, +dp/dtmax, heart rate and coronary flow were measured before and after storage, while lactate dehydrogenase, creatine kinase enzyme leakage and variety of pH were measuredafter reperfusion. Contents of adenosine triphosphate and wet weights in myocardium also were mearsured. Myocardial microstructural and ultrastructuralchanges were observed. Results The left ventricle function of UW and HCSgroups had slighter impairment than that of K-H group. Group UW and HCS showed a excellent recovery after 24h storage in HR(76.65±6.89, 78.92 + 7.18)%, LVPSP(79.97+4.67, 76.35 + 7.82)% and +dp/dtmax(73.72±4.25, 71.11+9.75). (P < 0.01 vs group K-H (38.66±4.82, 48.77±8.3K 30.70±5.67) %) and there are no significant difference between Group UW and HCS. Coronary flow was found to be significantly high in group HCS(76.35±7.82)(p<0.05 vs other two groups) and the group UW(47.99±6.71) was no statistically significant difference with K-H group(45.16 ±6.78). After reperfusion, the release of LDH and CPK in group UW and HCS were significantly lower than group K-H (p < 0.05) and the LDH and CPK concentration in group HCS was similar to the group UW (p>0.05). The ATP content of myocardium was markedly higher in group HCS(p<0.05) and was lowest in group K-H (p<0.05). The wet weight in myocardium was highest in group K-H(p<0.05,vs group UW and HCS) and no statistically significant difference was noted in other groups(p>0.05, group UW vs group HCS). Group HCS offer a less variety of pH (p<0.05, vs group UW ,HCS) and had a slightest acidosis. The time, from the reperfusion to beating of heart, was no difference between group UW and HCS. The microstructural and ultrastructural changes wereslight in group UW and HCS. Conclusion On the basis of these results, we conclude that HCS solution can proctect heart for 24h effectively and the recovery of heart function can reach to UW solution. HCS solution can prevent the ischemia/reperfusion injury and improve the coronary flow and acidosis. Also it is not only effective, but low cost ,convenient for blending , storage and transportation.
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