The Effects Study Of Fuwai Modified Solution, Histidine-Tryptophan-Ketoglutarate Solution And University Of Wisconsin Solution For Hypothermic Preservation Of Isolated Rat Hearts

Objective: In China, Histidine-Tryptophan-Ketoglutarate solution (HTK) and University of Wisconsin solution (UW) are often used for donor heart preservation. Howerver, reports conflict on the protective effects of HTK and UW solutions. Therefore, our objective was to assess the efficacy of several cardioplegic solutions for donor heart preservation by comparing HTK solution, UW solution and a solution from our laboratory, Fuwai Modified solution (FWM).Methods: We randomly divided male Wistar rats (n=20) to 4 groups: FWM, HTK, UW and Control. In the control group, hearts were harvested and immediately mounted on the Langendorff apparatus and reperfused. In the other three groups, the heart was rapidly excised and immersed in 1 of 3 preservation solution (4℃). The aorta was cannulated within 1 minute, and the cardiac coronary vessels were rinsed with the same solution for 3 minutes at a constant perfusion pressure (100 cm H₂O). We paid great attention to achieving strictly homogeneous initial...