The Expression Of Vascular Endothelial Growth Factor And P53 Protein And Their Relationship To Neoangiogenesis In Non-small Cell Lung Cancer

Background and Objective: Lung cancer is a maglinant tumor originated in lung.lt is one of the most common carcinomas in the entire world. In 1999 WHO reported that lung cancer is the leading cause of cancer death. It was thinked that oncogenes exert their effects on tumors through directly promoting proliferation and differentiation of tumor cells. Recent studies discovered that solid tumors require continuous vascularization for growth and metastasis. Studies showed that wild-type p53 could inhibit the expression of vascular endothelial growth factor (VEGF) and growth and metastasis of tumors but mutant p53 (mtp53) could up-regulate the expression of VEGF and promote vascularization. At the same time reverse conclusion was reported. In order to investigate the possible mechanism of lung cancer, a immunohistochemical method was used to examine the expressions of VEGF and mtp53 and MVD in 80 patients with non-small cell lung cancer (NSCLC).Methods The expressions of VEGF and p53 protein in 80 cases of non-small cell lung cancer tissues, 20 cases of benign lung tissues were examined by use of S-P immunohistochemistry. To calculate the number of microvessel labelled by anti-CD34 monoclonal antibody in lung cancer tissues. All samples had been proved by means of pathological diagnosis.Results (l)The positive rate of VEGF in lung cancers (64 of 80, 80.0%) was significantly higher (p<0.05) than that of VEGF hi benign tissues (3of 20, 15.0%). (2)No significant correlation was found between VEGF expression and age, gender, histologic type (p>0.05).(3)Significant differences (p<0.05) were found among the positive rates of VEGF expressions in high differentiation lung cancers (62.1%), intermediate differentiation lung cancers (88.5%) and low differentiation lung cancers (92.0%); The positive rate of VEGF in stage III-IV lung cancers (91.4%) was significantly higher (p<0.05) than that of VEGF in stage I ~ II lung cancers (71.1%) ; The positive rate of VEGF in lung cancers with lymphnode metastasis (90.9%) was significantly higher (p<0.05) than that of VEGF in lung cancers without lymphnode metastasis (66.7%). (4)The positive rate of p53 protein in lung cancers (48 of 80, 60.0%) was significantly higher (p<0.05) than that of p53 protein in benign tissues (0 of 20, 0.0%).(5)NO significant correlation was found between p53 protein expression and age, gender, histologic type (p>0.05). (6)Significant differences (p<0.05) were found among the positive rates of p53 protein in high differentiation lung cancers (41.4%), intermediate differentiation lung cancers(65.4%) and low differentiation lung cancers(76.0%);The positive rate of p53 protein in stage III-IV lung cancers (74.3%) was significantly higher (p<0.05) than that of p53 protein in stage I ~II lung cancers (48.9%); The positive rate of p53 protein in lung cancers with lymphnode metastasis (81.8%) was significantly higher (p<0.05) than that of p53 protein in lung cancers without lymphnode metastasis (33.3%).(7)A positive correlation was observed between the expression of VEGF and that of p53 protein (p<0.05). (8)MVD of lung cancers with positive expression of p53 protein (64.15 + 9.46) was significantly higher (p<0.05) than that of lung cancers with negative expression of p53 protein (42.61 ?7.36); MVD of lung cancers with positive expression of VEGF (59.76+11.66) was significantly higher (p<0.05) than that of lung cancers with negative expression of VEGF (38.49+5.38); MVD of lung cancers with simultaneously positive expressions of p53 protein and VEGF (66.34?.27) was significantly higher (p<0.05) than that of lung cancers with simultaneously negativeexpressions of p53 protein and VEGF (35.39+2.91).Conclusion (l)The positive rate of VEGF expression is high in lung cancer and strongly related to the degree of differentiation, TNM stage and lymphnode matastasis. Overexpression of VEGF may be closely related to lung carcinogenesis and development of lung cancer. (2)The positive rate of p53 protein expression is high in lung cancer and associated with...