Topographic Analysis On Expression Of C-myc,Rb,p16 And CD44v6 In The Whole Specimens Of Esophagectonmy

Carcinogenesis of esophageal carcinoma (EC) is considered a process of multifactor involving and multistage development. The development of EC involves some morphologic changes including basal cell hyperplasia (HP), clysplasia (DYS), carcinoma in situ (CIS) and invasive squamous cell carcinoma (SCC) and the molecular mechanism involved in this process is still unclear. Although more and more people study EC by various molecular biological methods, immunohistochemistry is still necessary and unsubstitutable. Moreover, getting materials with methods of only pots or from pots to lines may loss some important information in the extent. To further elucidate the possible mechanism of EC carcinogenesis, the present study was undertaken to observe the alternations of C-myc, Rb, p!6 proteins and expression of adhedsion molecular CD44 variant 6 (CD44v6) protein in the normal tissues and the tissues with different degrees of severity of 45 esophageal whole specimens from the patients with EC and carried out the topographic analysis for the expressions of these proteins in the whole EC specimens including the different lesions (normal mucosa , hyperplasia, dysplasia, carcinoma in situ and invasive carcinoma). It was to be analysed whether there were correlation between their expression and pathologic characters of esophageal carcinoma and to provide the theoretical basis and strategies for clinical biotherapy .Materials and Methods: 45 cases of surgically resected EC specimens werecollected from the First Affiliated Hospital of Zhengzhou University and PLA No.460 hospital. The whole of specimens were opened longitudinally and flattened, fixed with 10% formaldehyde, then several 0.5cm interval widely strips were taken on eachspecimen and given No.A, B, C......, these strips were cutten in 2.0cm granularityinto more pieces from top and given No. Ai,A2,A3,......,61,62,63,....... All pieceswere embedded in paraffin, and serially sectioned for morphology and immunohistochemistry (SP) analysis. For every specimen, a diagram showing the topographic location of cancerous and precancerous lesions was generated on the base of histologic results. The difference in immunoreactivity of C-myc, Rb, p16 and CD44v6 and their correlations with lesions progression were determined on diagrams. The chi-square test were used for the statistics. (p<0.05 was considered significant). Results:1. Of 45 specimens, 35 cases (77.8%) with serial lesions from HP, DYS, CIS to SCC were found, 7 cases showed HP and SCC lesions, 2 cases DYS and SCC, 1 case only SCC.2. Immunohistochemistic results:(1) Immunoreactivity for C-myc was located at the nucleus and cytoplasm, the rate ofpositive expression raised stepwisely (p<0.01), especially for cytoplasm- positive cases with the lesions progressing from normal epithelium to HP-DYS-CIS-' SCC. The expressions of C-myc (P=0.0026) were observed significant different between N and HP, furthermore cytoplasm-positive expression was significant correlation with clinical pathologic characters (p<0.05). No correlation for total positive expression of C-myc was found.(2) Immunoreactivity staining for Rb was located at the nucleus. Rb expressions werereducing stepwisely during multistep process of EC carcinogenesis (p<0.01). Furthermore, the expression of Rb was negative correlation with those of C-myc in SCC (p<0.01).(3) Dysregulation of p16 was observed during multistep process of EC carcinogenesis,and there was negative correlation between the expression of p16 and of Rb inDYS and SCC. Moreover no significant correlation with clinical pathologiccharacters was discovered. (4) Immunoreactivity for CD44v6 was chiefly located at the membrane and alsocytoplasm. The expression of CD44v6 was reducing stepwisely with esophageallesions processing carcinogenesis (p<0.01), and there was significant differenceobserved between CIS and SCC (P=0.048).The expressions of CD44v6 in SCCwere related to clinical pathologic characters(p<0.01). 3. These diagrams may i...