The Research For The Expression Of Interleukn 10,Tumor Necrosis Factor-alpha And Laminin In Human Placenta Of Pregnancy Induced Hypertension Syndrome

Preganacy-Induced-Hypertension syndrome(PIH) is a common serious pregnant complication in obstetric department.The incidency rate of it is 9.4%,it is an important cause of perinatal and maternal mortality. Because its etiology is undefined, it still be one of the most important subjects in perinatology by now.Abnormal remodeling vasculature is the characteristic patho;ogy in the placenta of PIH, it is also the crucial process of etiology of PIH and result from abnormal function of trophoblast(TB).Interleukin 10(IL-10) is a kind of new potent anti-inflammatory and immunosuppressor cytokine that can regulate various function of TB.It is mainly producted by villous trophoblast and could have paracrine effects on the maternal immune system to downregulate immunological tolerance.IL-10 improved the establishment of remodeling vasculature , placentaion and maturity of vessel web by stimulating the prolifertation, invasion of TB and decreasing the synthesis of pro-inflammatory cytokine.The mainly function of IL-10 is to regulate the balance of Thl/Th2 cytokine in vivo by suppressing the Th2 cytokine,such as tumor necrosis factor-alpha (TNF-a).Laminin is a importmant component of extracellular matrix and have important functions on stimulating the prolifertation, invasion of TB. So,the research for IL-10, TNF-a and LN is helpful to trace the reason of shallow invasion and clarify the etiology of PIH.The expression of IL-10 TNF-a and LN was measured by immunohistochemistry here,in order to elucidate the role ofIL-10, TNF-a and LN on the etiology of PIH,and then provide theoretical foundation of prevention and treatment.Material and Methods All subject were derived into three groups:(1)the early pregnant group (n=20),woman with normal pregnancy;(2)the normal late pregnant group (n=20); (3) the PIH group (n=58),according to the diagnostic standard (OBSTETRICS AND GYNECOLOGY, the fifth edit),it is former subdivide into mild (n=17),middle (n=20) and sever group (n=21).All patients were of without other complications of pregnancy and primary hypertension, renal disordes.(2)The biopsies were immediately collected after abortion or delivery, tissue was fixed in 10% formalin and embedded in paraffin wax for subsequent examination.(3)The expression of IL-10 , TNF-a and LN were determined using immunohistochemistry . Result (l)There are no significant difference between PIH group and the normal group in mean ages, gestational weeks and the basic blood pressure (P>0.05).The mean systolic and diastolic blood pressure of three group of PIH group were significantly higer than normal late pregnant group (P<0.05).The mean weight of newborns in sever PIH group was significantly lower than that of normal late pregnant group(P<0.05).(2)The strong immunostaining of IL-10 was observes in villous cytotrophoblasts and syncytiotrophoblasts in the placentas of normal early pregnancy.It also occurred in the villous cytotrophoblasts and syncytiotrophoblasts in the placentas of normal late pregnancy. The intensity of IL-10 immunostaining of trophoblasts in the placentas of late pregnant group was significantly lower than that of normal early pregnant group(P<0.05).(3)The intensity of IL-10 immunostaining of trophoblasts in sever PIH group was significantly lower than that of normal late pregnant group(PO.05),while it has no qualitative differences between the mild and middle PIH group and the normal group (P>0.05,P>0.05) .There was negative correlation between the intensity of IL-10 immunostaining of trophoblasts and the state of PIH(rS=-0.342 P=0.009).(4) The strong immunostaining of TNF-a was observes in villous syncytiotrophoblasts in the placentas of normal early pregnancy.lt also occurred in the villous syncytiotrophoblasts in the placentas of normal latepregnancy. The intensity of TNF-a immunostaining of syncytiotrophoblasts in the placentas of late pregnant group was significantly lower than that of normal early pregnant group(P<0.05).(5) The intensity of TNF-a immunostaining of syn...