| Objective:The cytokine has an effect of immunoregulation and immediate induction in the destruction pathology of periodontitis. In this experiment, the content of HGF and IL-1βin healthy periodontal sites and in periodontitis sites before and after periodontal treatment were measured by enzyme-linked immunosorbent assay (ELISA) . The relationship was discussed among HGF and IL-1βin GCF and clinical parameters. The purpose of this study is to research the relationship among HGF and IL-1βin GCF and periodontitis ;to discuss that HGF and IL-1βare regarded as beneficial indexes to reflect the periodontal status or not ; and to discuss the effect of HGF and IL-1βin periodontitis pathology and the illness progress.Methods:Subject selection:12 Subjects who had periodontitis in this study were selected from oral clinic in the Second Hospital of HeBei Medical University, including 7 male(range 31-43 years old) 5 female(range 35-45 years old) mean age 40.5 years old. Criteria for patients selection were : probing depth (PD)≥4mm,gingival index(GI)≥1,attachment level(AL)≥2mm,total 35 periodontitis sites. In every subject's mouth, 2-4 periodontitis sites that were not close together were selected.15 healthy subjects who had no periodontal disease were selected by random, including 8 male(range 28-35 years old),7 female(range 26-31 years old),mean age 31.75 years old. In every healthy mouth, only one site was selected, total 15 healthy sites. The healthy sites enrolled following criteria: probing depth (PD)<2mm, gingival index(GI)=0, attachment level(AL)=0mm. All subjects gave their informed consent to our investigation. They all met following orders: all subjects were free of systemic disease and had received no periodontal treatment for at least 6 months. They had not taken antibiotic, immunobiological, contraceptive drug at least in 3 months prior to examination. Women had no pregnancy. There are two groups in the experiment, one is control group and the other is experiment group. The collection and measurement of GCF: GCF was collected by filter paper strips. After collecting GCF samples, the clinical parameters such as probing depth, gingival index and attachment level were recorded. Four strips were used per site, two strips as a sample for one checked factor. Briefly, teeth were air dried and isolated with cotton rolls. GCF was sampled with precut filter paper strips(2mm×8mm). A filter paper strip were inserted periodontal pocket gently until you felt slight resistance. The strip was in the pocket for 30s.Then, placed it in an eppendorf (EP) tube immediately. If the strip stained flood, it would be discarded. The tube was placed immediately in a –70℃ refrigerator, after it was weighed with electronic balance. And then periodontitis patients took treatment of scaling and root planing and instruction in care for their oral health. These treatments were finished by one experienced doctor. Repeat the ways of collecting GCF above in 4 weeks after scaling and root planing. The determination of HGF and IL-1β:GCF samples were put together to analyze. The samples were taken out to refrozen in room temperature. The GCF-containing strip was immersed in corresponding assay buffer, extracted by vortex mixing and centrifuged at 1500g/minute for 3 minutes for use in the following assays. The HGF content of GCF was determined using a specific ELISA kit. The HGF content of GCF was immersed in 60ul of Calibrator Diluent RD 6X.50ul of each specimen was used for assay. IL-1βcontent of GCF was determined using a human IL-1βELISA kit. The IL-1βcontent of GCF was immersed in 110ul corresponding assay buffer.100ul of each specimen was used for assay. The assay progress according to the manufacturer's instruction.Results: HGF mean amount and mean concentration in periodontal sites before treatment were 4.78-fold and 1.24-fold higher than them in healthy sites. IL-1βmean total amount and mean concentration in periondontitis sites before treatment were 4.47-fold and 1.22-fold higher than them in healthy...
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