Studies On Fangfeng And Its Close Relative Species DNA Fingerprints And Quality Correlation

38 samples of five Species Fangfeng and Its Close Relative Species collected from Heilongjiagn, Hebei, Shanxi, Sichuan, and Gansu were analysed by RAPD and ISSR molecular markers. 21 RAPD primers and 9 ISSR primers were selected from 100 and 32 respectively, and could produced qualified fragments. Among these selected primers only 8 RAPD and 3 ISSR primers could identify the Fangfeng and Its Close Relative Species. DNA fingerprints were obtained by PCR with all selected primers and analyzed using cluster analysis. The fingerprints indicated that only few primers could get the specific fragments able to identify Fangfeng and Its Close Relative Species. The cluster analysis of cluster analysis based on the genetic similarity divide 38 samples of five Species Fangfeng to 3 groups: fisrt group included P.dielsianum Fedde ex wolff and Peucedanum ledebourieuoides K.T.Fu; second group included Curum Carvil and Friocycla albescens (Franch). Wolff; last group included Fangfeng. The genetic similarity of first two groups ranged between 0.77 to 0.80, the genetic similarity of first two groups and last group Fangfeng ranged between 0.72 to 0.75. The genetic similarity range of the different varieties show that The Genetic Distance of Fangfeng and Its Close Relative Species ,The genetic similarity of Its Close Relative Species,and also illustrate that The genetic similarity of Fangfeng and Its Close Relative Species were tiny, and relatively big between them.Content of prim-O-glucosylcimifugin, the mainly effective compound was determined by high performance liquid chromatography, the method used in People's Republic of China pharmacopeial convention. Also, the high performance capillary electrophoresis was used. People's Republic of china pharmacopeial convention prescript the content of prim-O-glucosylcimifugin in Fangfeng and Its Close Relative Species were aove 0.308. The Content of prim-O-glucosylcimifugin in Fangfeng selected from Heilongjiang Fuyu ranged from 0.842% to 0.864%,which obviously were much more than the Content in Its Close Relative Species,such as Friocycla albescens (Franch). Wolff(Hebei 0.159%～0.163%), P.dielsianum Fedde ex wolff (Sichuan 0.350%～ 0.411%),Peucedanum ledebourieuoides K.T.Fu (Shanxi 0.473%～0.590%),Curum Carvil(Gansu 0.456%～0.501%)。In concern of the DNA fingerprints, primer SBS A17 amplified a specific fragment within Fangfeng, while not in other samples.The selected primers could be used in identifying Fangfeng selected form HeilongjianFuyu produced the best quality Fangfeng containing highest content of prim-O-glucosylcimifugin, and should be considered first while culturing new good variety. The specific fragment amplified by primer SBS A17 could be used as a potential DNA marker indicating good quality of Fangfeng, especially useful in the use of identifying good seedling in an early stage of growth.