Effect Of Indomethacin On Human Laryngeal Cancer Hep-2 Cell Line

AIM:To assess the effect of indomethacin on tumor growth, invasion and induced -differentiation in human laryngeal cancer Hep-2 cell line in vitro.METHOD:1 .Hep-2 cell line was exposed to indomethacin at different concentration for 24h and 48h in vitro.Counted by trypan blue exclusion test to determine the viability of cells.The cell DNA analysis was conducted with flow cytometry.2.Hep-2 cells were exposed to indomethacin at different concentration for 48h.Then cell growth rate, the colonies formation in soft agar medium and cell mobility were examined. Monolayer invasion assay gave us cell invasion index.3.Cell morphology observation by phase contrast microscope, HE staining and transmission electron microscope, scanning electron microscope.RESULTS:1 .The alive cells after indomethacin treatment were reduced to a dose and tune-dependent situation. And cells appeared a significant peak of apoptosis in flow cytometry.2.Dealed with indomethacin,the colonies formed of Hep-2 cells in soft agar medium were fewer than that of control,the cell mobility was weakened and invasion index was decreased.But cells growth rate wasn't changed.3.After indomethacin treatment, Hep-2 cells were smaller and longer, strange nucleus rate decreased, microvillus and pseudopodium were less than that of control,especially in low-dose drug condition.CONCLUSION:1.Indomethacin could inhibit the growth of Hep-2 cell line.2.Indomethacin could inhibit the invasion of Hep-2 cells.3.Indomethacin might have the effect of induced-differentiation on Hep-2 cell line.