The Experimental Study On The Effect Of Salvia Miltiorrhiza Bge On The Seizure-induced Brain Injuries Of Ummature Rats

Objective:It has been reported that the incidence of status epilepticus in premature rats. The purpose of this study is to evaluate the protective effect of Salvia Miltiorrhiza Bge(SMB) on seizure-induced cerebral neuron injury of premature rats.Methods: Megimide(20mg/kg) was injected into healthy infant rats(21d) subcutaneously and intraabdominally to evoke status epilepticus (SE).Forty-eight premature rats were randomly devided into three groups: the SE group, treated with Salvia Miltiorrhiza Bge group and normal control group. Each rat of SE and SMB group was executed randomly after induced 72h,7d. Hippocampus neurons were observed with HE stain; Hippocampal neurons loss was observed with toluidine blue stain. The incidences of injuried neurons were counted at toluidine blue slices of hippocampus CA3,CA1,dentate gyrus and temporal cortex. The ultra-structure changes of the hippocampus neurons were observed under the electronic microscope. At the same time points, the level of NSE in rats sera was tested with ELISA..Results: Rats in the normal control group had no epileptic seizures, but both SE and SMB group had seizure of SE. By the time of 72h after the seizures, in the SE group, the arrange of hippocampal neurons in CA3,CA1 with HE stain were in disorder; polarity was not clear, vacuolization appeared, and the shape of cell nucleus was elliptic or rounded; with toluidine blue stain ,there were obvious loss of hippocampal neurons in hippocampus CA3,CA1, next to dentate gyrus, temporal cortex. In the hippocampus CA3 neurons of SE group rats, the mitochondria volume decreased, the matrix condensed, the ridge obscured or disappeared, and vacuoles formed in some mitochondria. Mild to moderate dilation of Golgis complex was also observed. By comparison, the SMB group had a lighter damage than SE group in above aspects. The normal control group had no similar damage of hippocampus CA3, CA1,dentate gyrus and temporal cortex. In the seventh day beyond seizures of SE, every damage of hippocampus neuron in SE group had alleviated a little than before. At the same time, the SMB group had a greater improvement than the SE group. The normal control group had no changes. The SE group rats in the 72h after seizures have a high level of NSE in sera, which is over the normal group at the same time after seizures. But the SMB group have a lower concentration of NSE than the SE group at the two time points.Conclusions: 1 There were a few brain neuron injuries of premature rats induced by status epilepticus, mainly in hippocampal CA3, following by hippocampus CA1, dentate gyrus, temporal lobe. 2 Basically, the same trend lies in both serum neuron specific enolase (NSE) concentration and brain neuron injury. The serum NSE could be an effective marker used to tell the existence of neuron injuries and point out the recovery.,however, which is limited by the period of seizures. 3 Salvia Miltiorrhiza Bge has a positive effect on the seizure-induced brain neuron injuries of premature rats, both in cellular and in biochemical levels. It provide the experimental proof for the clinical way to treat the seizure-induced brain injury.