| Objective:To investigate the effects of recombinant human epidermal growth factor (rhEGF) used at anastomotic site after gastrojejunostomy of rabbit on anastomotic wound healing and discuss its functionary mechanism. Methods: Japan big-ear rabbits were used for the experiments. Sodium Thiopental(20mg/kg) were used for intravenous anaesthesia. Gastrojejunostomy was operated on each rabbit (n=48) after laparotomy through epigastric midline incision, then all animals were divided into two groups. In experimental group, rhEGF were injected into the submucosa around the anastomotic site(2.0ml, 2000IU/ml). Venous blood of the two groups (n=8) were drawn out in day 1, 3, 5 postoperatively for lymphocytes counts and the measuring of serum C-reactive protein content. Rabbits were killed in pairs at day 3, 5, 7 postoperatively and relaparotomy were performed to observe the rate of the anastomotic leakage. The tissues at anastomotic site were removed for the measuring of hydroxyproline concentration and histological examination. Paraffin sections were cut and stained by haematoxylin-eosin and Masson. Collagen deposition was assessed by computer imagine analysis system. Results: There were no statistical different of the rate of anastomotic leakage, the peripheral lymphocytes counts and serum C-reactive protein content between the experimental group and the control group (P>0.05). The hydroxyproline concentration and collagen content of the tissue at anastomotic site significantly increased in experimental group compared with the control group in day 5, 7 postoperatively (P<0.05). Histological examination of the anastomotic site showed in the EGF-treated rabbits the area was more cellular than in the controls, showing an increasing in fibroblast , endothelial cells and capillary vessel in day 5 postoperatively. Collagen deposition also appeared to be increased apparently in day 3, 5, 7 postoperatively. Conclusions: Topical application of rhEGF can facilitate collagen synthesis to promote early anastomotic wound healing. But it can not change the immunity function and inflammatory response extent of the rabbits after gastrojejunostomy apparently. The effects of rhEGF on promoting early anastomotic wound healing are associated with its abroad biological functions: it can not only attract many kinds of inflammatory cells and tissue repairing cells to the injuried region as a powerful chemotaxin but also play a dominant role in the synthesis of collagen and non-collagenous protein to enhance tissue repair. Our results show that EGF is such a factor and represent another application for the clinical use to promoting anastomotic wound healing .Exogenous EGF may display its function more significantly if better preparation worked out and when used in individuals who have markedly impaired healing problems such as malnutrition.
|
PDF Full Text Request