Study Of The Protective Effects On Experimental Rat Hypertriglyceridemia And Its Primitive Mechanism Of Codonopsis Lanceolata

Codonopsis lanceolata (CL) is a kind of medical herbal roots, composed of many kinds of nutrient substances. This study was designed to examine the protective effect of CL on hypertriglyceridemia, which is known to be induced by a high-fat (primarily saturated) diet (HFS). First, male rats were fed with a well-balanced diet and given Codonopsis lanceolata ethanol (concentration 50%) extraction (CLEE) simultaneously for 8 weeks . The result indicated that the serum triglyceride (TG) in rats given CLEE have no statistical discrepancy compared with control group.And then preventive experiments using CLEE on hypertriglyceridemia rats were conducted. Control and model groups, fed with the well-balanced diet and high-fat one respectively, were given distilled water po. And experimental groups were given CLEE by 1.5. 3.0. 6.0g/kg bw po respectively and fed with high-fat diet. The results indicated that compared with control group, the serum TG in model group rats were increased significantly (p<0.05); and compared with model group. TG in rats given CLEE were decreased significantly (P<0.05). And Gypenosides group have similar effect with CLEE.To study the mechanisms intensively, a series of hypothesis were tested.As major enzymes responsible for hydrolysis of triglyceride molecules present in circulation. LPL and HL are associated with hypertriglyceridemia .The results showed that compared with control group, the activities of Upases, including LPL and HL, were significantly downregulated (P<0.05) in model group and compared with model group, were significantly upregulated in the medium and high doses groups (PO.05).The contributions of NO and NOS to atherosclerosis induced by hypertriglyceridemia were elevated. The results proved that CLEE inhibited NO level and eNOS activity lowering induced by hypertriglyceridemia significantly (TO.05).It is reported that MDA and GSH-Px are regarded as the sensitive indicates in hypertriglyceridemia study, which can reflect the metabolizability of antioxidants, so wedetected GSH-Px activity and MDA content of mitochondria and plasma of liver in rats. The results were that CLEE inhibited the decreasing of GSH-Px activity and the increasing of MDA content induced by hypertriglyceridemia. It was identified that CLEE can inhibit the generation of excessive lipid per-oxidation in liver mitochondria and supernatent (p<0.05).The effects of various dietary and their interaction with CL on several microelements (including Cu, Zn, Ca, Mg, Mn et al ) were tested. Among of them, especially cooper and zinc, thought to join in the body anti-oxidation, have practical significance to detect the element contents. It resulted that the contents of cooper and zinc in serum of model group were increased compared with control (p<0.05) and decreased in each doses groups in different degree (P<0.05). This indicates that CLEE has some effects on the metabolism of elements. And other elements contents had not significant changes.Above all. it suggested that Codonopsis Lanceolata had protective effects on hypertriglyceridemia, and the mechanisms were complex.