An Experimental Study On The Cold Preservation Of Rat Liver With Self-designed KYL Solution

Objective To compare the effects of self-designed KYL solution on rat liver preservation with UW solution. Mdthods Using non-circulated isolated perfused rat liver (IPRL) ,Wister rat livers were randomly preserved for 0, 4, 8, 16, 24and 48h with self-designed KYL solution or UW solution. The effects were assessed by measuring the bile production, the contents of AST, ALT, LDH and free radical in Krebs-Henseleit' s perfusate and the content of intracellar calaium of the rat hepatocytes, and by observing the hepatocellular apoptosis and the related-genes' expression and the morphological changes in liver. At the same time , to know KYL solution and UW solution can prevent the ischemia-reperfusion injury in rat liver transplantation , set up the normal saline as the negative comparison to preserve rat livers. Results At different periods of preservation, the bile production of the rat livers which were preserved by KYL solution were more than those were preserved by UW solution , and the content of intracellar calaium of the rat hepatocytes which were preserved by KYL solution were less than those were preserved by UW solution . The contents of AST> ALT, LDH , MDA and SOD in Krebs-Henseleit' s perfusate , the percent of apoptotic cells and the expression of the apoptosis- related-genes were resemble in the rat livers preserved by both KYL solution and UW solution. Morphological changes in both proups were almost same. All variables of both proups were better than the rat livers preserved by the normal saline. It proved that both KYL solution and UW solution could prevent the rat livers . Conclusion The study indicated that the effects of self-designed KYL solutionon on rat liver preservation were similar to that of UW solution. The bile production after reperfusion and the content of intracellar calaium of the rat hepatocytes in KYL group were better than UW group .