The Pathological Study Of The Influence Of BPH On The Serum PSA Rising

Baekground: Benign Prostatic hyPerplasia(BPH)15 a common disease in male more than 50 yearsold,and eareinoma of Prostate(PCa)15 also easy to haPPen in the erowd.Therefore,earlydiagnosis and differential diagnosis are imPortant significanee.From the 10 years reeently,Prostate sPecfie antigen(PSA)in blood serum has already been the widest mark of PCa inclinieal study and aPPlication,whieh have the sensibility and sPeeifieity for PCa screeningand whieh are eorresPonding elinieal value to judging PCa size,staging and grading,toseleeting PCa treatment,to judging therapeutie effeet and to estimating and monitoringPfogn0SIS. The serum PsA of the common ma一e 15 less than 4 ng·ml.l,whose diurual variation 15tiny.BPH and PCa,5 serum PSA are rising,whose age with disease are in eonformity.Theirexcreting PSA ways are also different,which make the serunn PSA level disParated.80percents of Pea serum PsA in elinic are more出an 4 ng·ml'1.HowevertheserumPsAofBPH Patients are lighely rising because of their gland ePithelial tissue hyPerblastosis andvolume augmentation Primarily exeePt for their ages,and the major are in normal range(<4ng·ml'l).But the serum PsA ofthe partial ones are rising disparitly.Aeeording to Hudson;srePorts, there are about 2 or 3 Pereents of the Patienis that their serum PSA are more thanlong·ml'1,whieh eouldn,t be enough to be explained only on the above一mentionedPhysiologie factor'5 Point of view.And now there are not eorrelated rePorts. ExcePt the above一mentioned factors that are able to effect the serum PSA as yet,androgen and ejaeulation also may effect the density of PSA.The iatrogenie factors,forexamPle,Prostatic massage,Prostate bioPsy and TURP,may make the serum PSA riseobviously.In addition,the Per reetum tyPe一B ultrasonie,eystoscoPe eheek,uroschesis andbacteral Prostatitis ete,maybe influence the lever of PSA.Among the above一mentionedfactors excePt for the Physiologic ones,Prostatitis can Persistently effect the environment ofProstate gland.Then whether or not that 15 the reason of the serurn PSA rising that BPHPatients are with Prostatitis? In view of the requirement to diagnose,differential diagnosis and treatment of PCa,raising the PSA'5 sensitivity and sPecifieity as the tumorous mark,the toPie analyzes andstudies the 1 72 BPH patients'prostate specimen sliees whieh have been verificated onPathology.Form this we hoPe that we ean find out the reason of the BPH Patienis'serumPSA rising and investigate the meehanisms. objeetives The toPie will analyze and study the 1 72 BPH Patienis'Prostate exairesis sPeeimensliees whieh have been verifieated on Pathology,HE dyeing and PSAimmunohistoehemistry dyeing utilized.Then we ean find out the variation of BPH Patients'Prostate gland when the serurn PSArising,and ehanges of PSA seeretion and exPression onProstate,from whieh we eould investigate the etioPathogenisis and the meehanisms. Methods: l,HE dyeing Sliee deParaffinaged in xylol,hydrated in gradieni aleohol,dyeing in Lillie Mayerhematoxylin staining solution,differentiated inl%liquor of hydroehlorie aeid and aleohol,flushed with water,dyeing eosine staining solution,flushed with water again,seParatingeolor.gradieni aleoholie dehydrated,the mixed liquor of fenol and xylol eleared,neutralgurn mounied. 2,PSA dyeing 10%buffering neutral formalin fixed;routine arthydration,elearing,immerse wax,embedding;1 .5一2 p m slice stretehed,sliee deParaffinaged in xylol,hydrated in gradienialcohol,dealed with3%hydrogen Peroxide,rinsed with PBS,antlgen restored,rinsed withPBS again,attached with Polylysine,negative eontrolled with PBS rePlaeed of monantigen.Dealed with mierowave oven for 1 0 minutes in 92一98oC,monantigen and diPlantigendroPwised,rinsed with PBS again,eolourated with DAB,afterstained with hematoxylin.routine dePrivated,elearing,mounting.二3 the serum PSA detectionDetection with radio...