| Background and Objectives Pulmonary thromboembolism(PTE) is the most frequent type of pulmonary embolism(PE), and it occupies a large part of PE. Acute PTE is thought as one kind of severe disease because of high mortality, misdiagnostic rate and feature of rapidness. In China, PTE was ever considered as a rare disease, and in a great extent resulted in ignoring to identify and diagnose it in medical realm, however, PTE is an frequent one actually. In recent years, data from some Chinese hospitals has shown that morbidity of PTE had an increasing trend. The rate of misdiagnostic PTE is possible 80% by estimatation, so it has severe negative effect on treatment and prognosis. Therefore, it has great significance to establish animal model of acute PTE to improve the diagnostic and treatmental technology. Recently, medical scholars have proved that acute PTE can triggered coagulative system and thrombolytic system simultaneously. More scholars began to pay attention to effect of prostacyclin(PGI2) and von Willebrand factor(vWF) among lots of activated factors.However, most study on PGI2 and vWF was about cardiovascular and hematologic disease. In the present study, we consecutively investigated the level of PGI2 and vWF on different time points before and after acute PTE model of rabbits by injection ofautologous blood clots stabilized in 70 C of distilled water through the femoral vein. Thus we tested function of those two factors in PTE, and tried to present new thoughtsthat may help diagnosis or treatment.Materials and methods: Thirty rabbits were randomed to either PTE group (n=15) or control group (n=15). PTE model of rabbits were established with injection of autologous blood clots stabilized in a temperature-controlled (70C) of distilled water for ten minutes through the femoral vein . 2ml blood was collected from earlap vein of rabbits periodically. Either 0.9ml was put into a sterile testtube which had 0.1ml 0.109mol/L tri-sodium citrate respectively. Blood samples were discentered for ten minutes at 3000 rotates per minute under temperature 4 C, then were stored in temperature -80 C in refrigerator. The PTE models of rabbits were explored at dissection and upon microscopical examination after they were killed on the 10th day. The plasma levels of PGI2 and vWF were examined by ELISA . The data were analysed by software SPSS 10.0. T-test was used to compare the difference between two groups and repeated measures ANOVA was used to compare between before and after PTE. P value < 0.05 is considered as statistically significant and P <0.01 is considered as much more significant statistically.Results1. The 15 rabbits of PTE group all had symptoms including increase of respiratory, tachycardia, panting, dysphoria, etc. The 15 of control group were normal.Pathologic studies: In lungs of PTE group thrombus can be find in pulmonary arteries, and secondary thrombus can be seen around thrombus, some arteries convulsed apparently. The lungs of control group are normal.2. The plasma level of PGL: In PTE group there was very significant statistical differences between before and after PTE(P<0.001): After PTE, it began to go up at 30min rapidly and highly, then went down to minimum on 1th day and remained lower level till 10th day though it went up very slowly. In control group there was notsignificant difference (P>0.05). Comparisons between groups: There were statistical significance on point, 30min, 1th day, 3th day, 5th day.3. The plasma level of vWF: In PTE group there was very significant statistical differences between before and after PTE(P<0.001): After PTE, it began to go up at 30min, reached maximum on 1th day, then remained higher level till 10th day though went down back gradually. In control group there was not significant difference (P>0.05). Comparisons between groups: There were statistical significance on point 30min, llh day, 3th day.Conclusions1. It has scientificity and feasibility to establish acute pulmonary thromboembolism model of rabbits with injection of autologous blood...
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