Expression Of Survivin,P53 And Bcl-2 In Non-Small-Cell Lung Cancer And Their Significance

[Background and Objective]Lung cancer is a maglinant tumor ,which does harm to humankind severely, and both the morbidity and mortality of lung cancer rise in the world. In the developed countries and metropolises of China,its mortality was on the top list of tumors. Although interventions such as operation,radiotherapy,chemotherapy and biological therapy were used,there was no obivious improvement in prognosis ,for the devolopment of lung cancer was a complicated course with multi-gene change. It was thinked that the activation of oncogene and the inactivation of antioncogene were the molecular basis of the forming of tumors, which disturb the normal regulation of the cell proliferation and differentiation.lt was showed that disorder of apoptosis was also common in tumor agenesis. Apoptosis was important to the stability of the internal environment of the body .The chaos of the regulation of apoptosis contributed to cell proliferation and the development of tumors,which was one of important mechanisms in the forming of tumor. Survivin was a novel inhibitor of apoptosis protein. The distribution of Survivin had a high selectivity. There was no Survivin expression in normal tissues (except pectoral gland and genital gland) but in many tumors. Survivin expression had correlation with the patient's prognosis. P53 and Bcl-2 ,which had a close relationship with tumor agenesis , were correlative with apoptosis and had beenwidely studied. At present, there were few papers about Survivin expression in lung cancer and it was not clear about the correlation between its expression and clinical pathology. There were no reports about the relationship between Survivin expression and P53, Bcl-2 in China. In order to investigate the possible mechanism of lung cancer, the immunohistochemical method was used to examine the expression of Survivin,P53 and Bcl-2 in 80 patients with non-small-cell lung cancer (NSCLC).[Materials and methods]Eighty surgically resected non-small-cell lung carcinoma tissues were collected. Among them male 55, female 25; 45 cases were Squamous cancer, 35 cases were adenocarcinoma; In addition, 16 cases were Well-differentiated Squamous cancer, 15 cases were moderately-differentiated Squamous cancer, 14 cases were poorly-differentiated Squamous cancer, 25 cases were Well-differentiated adenocarcinoma, 10 cases were poorly-differentiated adenocarcinoma; and there were lymph nodes metastasis in 44, and no lymph nodes metastasis in 36; 20 cases inflammatory lung lesions were collected as control groups. All the tissues were fixed in 10% neutral formalin and embedded in paraffin. SP immunohistochemical method was performed to detect the expressions of Survivin, P53 and Bcl-2. The data were analyzed by software SPSS 10.0, Chi-square Test and exact probability method were used to compare the difference of Survivin, P53 and Bcl-2 expression between groups. a=0.05 was considered as the level of tests.[Results]1. The positive rate of Survivin in lung cancers (61.3%) ,was significantly higher than that of Survivin in benign tissues (0.0%) (p<0.05); The positive rate of Survivin in stage III- IV lung cancers (72.0%) was significantly higher than that of Survivin in stage I -II lung cancers (43.3%) (p<0.05); No significant correlation was found between positive Survivin exprssion and age ,gender, histologic type ,tumor differation and lymphnode metastasis (p>0.05).2. The positive rate of P53 in lung cancers (55. 5%) , was significantly higher than that of P53 in benign tissues (0.0%)(p<0.05); The positive rate of P53 in stageIII-IV lung cancers (70.0%) was significantly higher than that of P53 in stage I ~ II lung cancers (30.0%) (p<0.05); The positive rate of P53 in lung cancers with lymphnode metastasis (70.5%) was significantly higher than that of P53 in lung cancers without lymphnode metastasis (36.1%)(p<0.05); No significant correlation was found between positive P53 expression and age, gender, histologic type and tumor differation (p>0.05).3. The positive rate of Bcl-2 i...