Correlation Between Expression Of Survivin And Colorectal Tumor

Objective: To identify the role of surviving novel inhibitor of apoptosis(IAP) in colorectal tumorigenesis and its correlation with angiogenesis of colorectal tumor by means of investigating tissue expression of survivin,VEGF and CD34 in human colorectal tumors including 11 hyperplastic polyps,23 adenomas with low dysplasia,20 adenomas with high dysplasia and 29 carcinomas in adenoma. Methods: Immunohistochemical staining for the paraffin sections by using the polyclonal antibodies of survivin, monoclonal antibodies of VEGF and CD34, was performed by the standard avidin-biotin-peroxidase technique. The percentage of positive cell and quantitative analysis were made by image analysis system, which also helped to calculate the amount of MVD. Results: 37 out of 65 cases of colorectal carcinomas were found expressed positively. The expression of survivin in the tissues of colorectal carcinomas showed no obvious correlations with tumor size, tumor invasive depth, Dukes stage and lymph node metastasis (p<0.01 for all). The immunoreactivity of survivin significantly increased in the transition from adenoma with low dysplasia to adenoma with high dysplasia (p<0.01 ) Similar changes in protein expression were observed for VEGF and MVD. The expression of survivin was closely related with that of VEGF . Both were positively correlated with MVD. Conclusion: Survivin plays an important role in the transition from adenoma with low dysplasia to high dysplasia during human colorectal tumorigenesis. Survivin promotes angiogenesis of colorectal tumor together with VEGF. VEGF may be the potential causes of reexpression of survivin during colorectal tumorigenesis.