Studies On Constituents In The Root Of Salvia Przewalskii Maxim.

The crud drugs of Salvia miltiorrhiza Bunge (Danshen) and related plants have effects on removing stasis and alleviate pain, promoting blood circulation and promoting menstruation, clearing away heart-fire to relieve vexation. Salvia przewalskii Maxim, is a plant distributed in southwest and northwest China, where it is habitually used as one of most commonly local species using as Danshen. But its constituents were still poorly studied. To ensure the safety and effectiveness of this crud drug, we investigated systematically its constituents, and bioassayed its anti-myocardial ischemia compound.15 compounds were isolated from the EtoH(80%) extract with column chromatography of silica gel, macroreticular resin, sephadex LH20, polyamide, reverse-phase chromatography and HPLC, and with experimental model of myocardial ischemia induced by ligating the coronary artery of dog .The compounds were identified on the basis of spectral analysis (UV, IR, MS, NMR, 2D-NMR) and phytochemical properties, These compounds identified as:t anshinone A, crypotanshinone, przewaquinone A, sugiol, tetracosyl feralate, ursolic acid, 2a,3a-dihydroxy urs-12-ene-28-acid, oleanolic acid, neo-przewaquinone A, protocatechaldehyde, protocatechuic acid, caffeic acid, ethyl-1-O-D-glucopyranoside, rosmarinic acid, lithospermic acidB. Among these compounds ,the compound is a new compound, compound, are new natural product, compound, are isolated from this plant for the first time.The obtained active compounds having effect of anti-myocardial ischemia includes rosmarinic acid, lithospermic acid B, protocatechaldehyde, protocatechuic acid, caffeic acid and ethyl-1-O-D-glucopyranoside.The contents of rosmarinic acid and lithospermic acid B in Salvia przewalskii Maxim, and 14 species plants (Labiatae) were determined by HPLC. The result show that the contents of lithospermic acid B in the plants are low. The contens of rosmarinic acid in 8 plants are high, which are Ajuga ciliata Bunge, Perilla frutescens (L.) Britt.(Baisu), Perilla frutescens (L.) Britt.(Zisu), Salvia przewalskii Maxim., Mentha spicata L., Salvia plebeia R.Br., Mentha haplocalyx Briq.(Zhouyebohe), Mentha haplocalyx Briq. The content .especially in Salvia plebeia R.Br. is over 8%, which means that the plant may be used as source of rosmarinic acid.