Three Compounded Injections Made From Sanqi And Three Salvias Native To Yunnan, China Protecting Myocardium From Ischemia/Reperfusion Injury In Rats

Background: Danshen (Salvia miltiorrhiza Bunge, SM), Diandanshen (S. yunnanesis C. H. Wright, SY), Ganxishuwei (S. przewalskii Maxim., SP), Hemaoganxishuwei (S. przewalskii Maxim. Var. mandarinorum stib.), and Sanqi [Panax notoginseng (Burk.) F.H.Chen, PN] are traditional drugs, which have been used to treat cardiovascular diseases for many years in China. Objective: In order to find out the cardio protection induced by three compounded injections made from SY+PN, SP+PN, SPM+PN respectively, during ischemia/reperfusion in rats, compared with injection made from SM+PN. And to confirm the synergism of the compounded injections, and find out whether the three drugs, SY, SP, and SPM can be used as SM against cardio ischemia/perfusion injury or not. Methods: Two ischemia/reperfusion models were employed. In one mordel, we ligated rats' artery of left anterior descending (LAD) for 30 min, then let it loose for 60 min, and we recorded the electrocardiogram (ECG) during experiment. We examined maleic dialdehyde (MDA), lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in rats' serum after reperfusion. In another model, a heart-perfusion model in vitro, we perfused every rat's heart at low rate of flow with Kreb's solution for 15min at first, then followed normal perfusion for 30min, also examined MDA, LDH, CK, SOD, and GSH-Px in the flow of effluent after reperfusion. Contractile force, perfusion flow, and rhythm were recorded during experiment. Results: 1) All the four injections were able to decrease MDA, CK, LDH, and increase SOD, GSH-Px, in a way of dose-dependence, in vivo and in vitro. 2) All the four injections were able to quicken heart rhythm, mitigate ST segment, and decrease infarcted area in vivo. And were able to alleviate contractile force during ischemia, and enhance myocardio constractile force, increase coronary flow in the state of reperfusion. 3) It was indicated that all the compounded injections decreased CK in vivo more powerfully than all the single historical ones respectively. 4) The three compounded injections of SY, SP, SPM decreased MDA at high dose (4g/kg) more powerfully than that of SM (p<0.05,0.01), in vivo; and except for SY, increased GSH-Px more powerfully than that of SM (p<0.05). In vitro, at high dose, the compounded injection of SP more powerfully increased GSH-Px than that of SM (p<0.01). Conclusion: All the three compounded injections (from SY+PN, SP+PN, and SPM+PN, respectively) employ cardio protection against ischemia/perfusion injury, and are similar to injection from SM+PN, and are more effective than the single ones respectively, which suggest that there is synergism of the compounded injections and that the three drugs can be replacer for SM to protect myocardium from ischemia/reperfusion injury. The mechanisms of which may be associated with blocking calcic channels, deactivating oxygen free radicles.