| Objective:1. To observe the effect of alcohol diet on rabbits' cardiac cell under optical microscope, and the treatment efficiency of vitamin C and coenzyme Q10 .2. To observe the effect of alcohol diet on NO, NOS, MDA in rabbits blood plasma, and the treatment efficiency of vitamin C and coenzyme Q10.3. To observe the effect of alcohol diet on expression of proto-oncogenge C-myc and C-fos in rabbits' cardiac cells, and the treatment efficiency of vitamin C and coenzyme Q10.4. To observe the effect of alcohol diet on the ultrasonic parameters, such as LVPWd, LVSd, LVDd, FS%, EF%, E, A and E/A of rabbits heart, and the treatment efficiency of vitamin C and coenzyme Q10 -5. To study the significations and relationships of the blood plasma enzyme, heart ultrasonic parameters and expressions of cardiac cell proto-oncogene after alcohol diets.6. To study the protection to cardiac muscles of vitamin C and coenzyme Q10 under the existence of alcohol, and to explore the mechanism of them.Methods:1. Groups:30 white otter rabbits were randomly divided into three groups: group I (the control group): Echocardiographs were detected and serum species were examined after fed with normal diet for 30 days, then put to death and pathologic examinations were performed; group II (the alcohol group): Echocardiographs were detected and serum species were examined after fed with alcohol for 30 days, then put to death and pathologic examinations were performed; group III (the treatment group): Fed with alcohol for 30 days, treated with vitamin C and coenzyme Q10 for another 15 days, echocardiographs were detected and serum species were examined, then put to death and pathologic examinations were performed.2.Ultrasonic parameters examination:The rabbits were anesthetized with 25~30mg/kg sodium pentobarbital intravein, lying on back. Limbs were fixed onto a wooden plate. The wooden plate was sloped to left at angles for about 30. Cleared the chest hairs, used the probe head with frequency at 3.0 MHz. The instrument plus was fixed at 50dB, and the image depth was regulated to 2.5cm.Put the probe head in the left of the sternum, displayed the left ventricular long axis, and detected. Each ultrasonic value was taken the average value of 3 cardiac cycles, and the ultrasonic examination was according to the standard determined by the ultrasonic association of America.3.Observation under optical microscope:Eviscerated the myocardium of post wall and intermediate septum of rabbits respectively, HE stain, observed the morphological changes under optical microscope.4.To observe the expressions of myocardial cellular proto-oncogenges:Ten rabbits were selected from each group, and their heart were taken out immediately when killed, heart tissues were fixed in 10% formaldehyde aqueous solution for 72 hours. Then the heart tissues were dehydrated, transparentized , embedded into paraffin wax, and cut into slices with 4um thickness along cross section and vertical section. Five slices were collected in each section and the thirdwere dyed with HE. After the heart tissues were confirmed to be ventricular tissues according to the size of cells, the color of cells, the area of nucleus and the area of plasmas under optical microscope. The slice was selected to do immunohistochemical examination.5. Measured the contents of NO, NOS, MDA in blood plasma with spectrometer. Results:1. group II had a higher injury on geometric patterns pathologically under optical microscope than group I and groupIII. groupIII had a higher injury on geometric patterns pathologically under optical microscope than group I .2. The contents of NO and NOS in blood plasma of group II were lower than that of group I and group III (P<0.01, P<0.05), while group III were lower than groupI (P<0.05).3. The content of MDA in blood plasma of group II was higher than that of groupI and group III (P<0.01, P<0.05), while group III were higher than group I (PO.05).4. The expressions of myocardium proto-oncogenes of C-myc and C-fos in groupII were significantly higher than that...
|
PDF Full Text Request