Qi And Phlegm And Blood Circulation Of Shr Myocardial Remodeling And Related Proto-oncogene Expression

Effect of treatment of the thcrapic principle (invigorating Qi, removing the phlegm and promoting blood circulation )on the remodeling of myocardium and the expression of proto-oncegene in spontaneously hypertensive ratsLeft ventricular hypertrophy(LVH) was one of the main complications of essential hypertension(EH), and was considered as an independent cardiovascular dangerous factor in these years. The tissue of left ventricular remodeled, comprising the hypertrophy of cardiomyocyte and the fibrosis of interstitial of heart. Blood stream dynamics, nerve factors, body fluid factors, and internal secretion factors have been involved in the occurrence and development of essential hypertension with LVH. During these years more and more scholars have found that cardiovascular diseases are connected with proto-oncogene closely. LVH was considered as the responsive reaction of cardiomyocyte and the interstitial cells of heart to the growth factors. With the aggravation of the burden of heart, the expression of cellular proto-oncogene c-myc, c-fos and ras were enhanced abnormally, which made cardiomyocytes multiply and hypertrophic. And LVH might be the finally result of some proto-oncogenes which had been activated.With the development of hypertrophy of left ventricular, the diastolic stiffness increased and the left ventricular compliance decreased. This could make the ventricular function lower, and the symptom of heart failure would be found lastly. The abnormal accumulation of interstitial collagen and myocardial fibrosis could destroy the network structure of myocardial interstitium. So the probability of the occurrence of arrhythmia and sudden death would be arising sigr~ificantly. To treat the EH, it is not only lowering the blood pressure to ordinary range, but also reducing the dangerous factors which were arisen by EH-LVI-I, such as sudden death, arrhythmia, myocardial infarction, and so on. The result was reversing the cardiac hypertrophy and improving life quantity. The current therapy of western medicine was fairlygood at lowering blood pressure and left ventricular mass index, but not quite good at recovering the course of fibrosis to improve heart function.It could be discovered that most of the patients of EH-LVH were middleaged and the course of the disease was long as well as the disease was often recrudesced. In accordance with the clinical symptoms and basic theory of TCM, we think LVH was caused by the accumulation of phlegm and blood stasis in the heart. Both excess syndrome and deficiency syndrome were the two sides of the disease. So combination of strengthening the body resistance with removing pathogenic factors which can be applied to syndromes of deficient vital-Qi with excessive pathogenic factors. Such therapic principles as invigorating Qi, removing the phlegm and promoting blood circulation were adopted. XingJiKang(XJK)pill was made for the patients with EH-LVH, the clinical study previously indicated that XJK had particularly curative effect , and could improve the life quality.The further experiment ~vas made and spontaneously hypertensive rat(SHR)was used, systolic blood pressure was measured by tail-cuff method .LVH was defined according to the ratio of left ventricular weight to body weight. Specific surface of myocardial mitochondria membrane was evaluated by the result of electron microscope. The expression of cellular proto-oncogene N-ras was calculated by PCR. Conclusions showed that the traditional Chinese medicine could reduce the systolic blood pressure(SBP), left ventricular weight(LVW) of SHR, and increase specific surface of myocardial mitochondria membrane( 6 s), it also could restrain the enhanced expression of cellular proto-oncogene N-ras in hypertrophy myocardiocyte.