The Experimental Study On Myocardial Injury Of Rabbits Fed With Alcohol And Treated With Trimetazidine

Objective:1.To observe the effect of alcohol diet on rabbits'cardiac cell under optical microscope, and the treatment efficiency of trimetazine.2.To observe the effect of alcohol diet on NO, SOD, MDA in rabbits blood plasma, and the treatment efficiency of trimetazine.3.To observe the effect of alcohol diet on expression of proto-oncogenge C-myc and C-fos in rabbits' cardiac cells, and the treatment efficiency of trimetazine.4.To observe the effect of alcohol diet on the ultrasonic parameters, such as LVPWd, LVSd, LVDd, FS%, EF%, E and E / A of rabbits heart, and the treatment efficiency of trimetazine.5.To study the significations and relationships of the blood plasma enzyme, heart ultrasonic parameters and expressions of cardiac cell proto-oncogene after alcohol diets.6.T0 study the protection to cardiac muscles of trimetazine under the existence of alcohol, and to explore the mechanism of them. Methods:1 Groups:30 rabbits were randomly divided into three groups: group I (the control group): Echocardiographs were detected and serum species were examined after fed with normal diet for 30 days, then put to death and pathologic examinations wereperformed;group II (the alcohol group): Echocardiographs were detected and serum species were examined after fed with normal diet and alcohol for 30 days, then put to death and pathologic examinations were performed;group III (the treatment group): Fed with normal diet added to trimetazine and alcohol for 30 days, echocardiographs were detected and serum species were examined, then put to death and pathologic examinations were performed.2 Ultrasonic parameters examination:The rabbits were anesthetized with 25 ~ 30mg / kg sodium pentobarbital intravein, lying on back.Limbs were fixed onto a wooden plate. The wooden plate was sloped to left at angles for about 3 0. Cleared the chest hairs, used the probe head with frequency at 3.0 MHZ.The instrument plus was fixed at 50dB, and the image depth was regulated to 2.5cm. Put the probe head in the left of the sternum, displayed the left ventricular long axis, and detected. Each ultrasonic value was taken the average value of 3 cardiac cycles, and the ultrasonic examination was according to the standard determined by the ultrasonic association of America.3 Observition under optical microscope:Eviscerated the myocardium of post wall and intermediate septum Of rabbits respectively , HE stain, observed the morphological changes under optical microscope.4 To observe the expressions of myocardial cellular proto-oncogenges:Ten rabbits were selected from each group, and their heart were taken out immediately when killed, heart tissues were fixed in 10%formaldehyde aqueous solution for 72 hours. Then the heart tissues were dehydrated, transparentized, embedded into paraffin wax, and cut into slices with 4μm thickness along cross section and vertical section. Five slices were collected in each section and the third were dyed with HE. After the heart tissues were confirmed to be ventricular tissues according to the size of cells, the color of cells, the area of nucleus and the area of plasmas under optical microscope. The slice was selected to do immunohistochemical examination.5 Measured the contents of NO, SOD, MDA in blood plasma with spectrometer. Results:1 .Group II had a higher injury on geometric patterns pathologically under optical microscope than group I and group III. Group III had a higher injury on geometric patterns pathologically under optical microscope than group I.2.The content of NO in blood plasma of group II was lower than that of group I and group III(P<0.0 1, P<0.05), but there is no obvious difference statistically between group I and III.3.The content of MDA in blood plasma of group II was higher than that of groupI and group III(P<0.0 1, PO.05), while group III were higher than group I (PO.05).4.The content of SOD in blood plasma of group II was lower than that of group I and group III(P<0.0 5, PO.05), but there is no difference between group I and III. 5.The expressions of myocardium proto-oncogenes of C-myc and C-fos in groupII were significantly higher than that in group I and group III, while group III were higher than group I.6.The ultrasonic parameter values such as EF %, FS %, E and E / A of group II were obviously lower than those of group I and group IIL The value of LVPWd, LVDd and LVSd was obviously higher than those of group I and group III. Apart from LVPWd > EF% and FS%,the other ultrasonic parameters of group III and group I had no obvious differences statistically.7.Ultrasonic parameters such as LVPWd, LVDd and LVSd had positive correlations with C-myc and C-fos respectively. EF%, FS%, E and E/A had negative correlations with C-myc and C-fos. Ultrasonic parameters such as LVPWd, LVDd and LVSd had negative correlations with NO and SOD., EF%, FS %, E and E / A had positive correlations with NO and SOD.Ultrasonic parameters such as LVPWd, LVDd and LVSd had positive correlations with MDA. EF%, FS%, Eand E/A had negative correlations with MDA. Conclusions:1.Alcohol call cause the cardiac injuries in rabbits , such as inflammation, hemorrhage, myocardial cellular apoptosis and cardiac fibrosis and so on.2. Alcohol can decrease the contents of NO in blood plasma of rabbits.3.Alcohol can increase the content of MDA in blood plasma of rabbits.4. Alcohol can decrease the contents of SOD in blood plasma of rabbits.5.Alcohol can enhance the expressions of myocardial cellular proto-oncogenes C-mycandC-fos.6. Alcohol can decrease the systolic function and diastolic function of heart.7.Under the existence of alcohol, the ultrasonic parameters of heart, the contents of enzyme in blood plasma and the expressions of proto-oncogenge of rabbits were closely related. Alcohol can decrease the activity of NO and SOD, enhance the reaction of lipid peroxidation, induce the expression of proto-oncogenes of C-myc and C-fos, induce the degeneration, apoptosis of myocardial cells and make fibrosis of cardiac muscles. The latter two factors were the molecular biological foundation of changes of heart geometric patterns, which belonged to alcoholic cardiomyopathy. Proto-oncogenes of C-myc and C-fos may be initiative factors of alcoholic cardiomyopathy.8.Trimetazidine have protective effects in alcoholic cardiomyopathy.The mechanism may be related to recreasing the activities of NO and SOD in blood plasma of rabbits, delaying the ruination of oxygen free radicals, inhibiting the peroxidation reactions,suppressing the early expressions of proto-oncogenes of C-myc and C-fos,thereby relieving the myocardial cellular degeneration, apoptosis and myocardium fibrosis, recoverying the decreases of cardiac function.