Expression And Ignificance Of PTEN And MAPK In Bladder Transitional Cell Carcinoma

PrefaceIt is thus clear that Bladder transitional cell carcinoma is the most common malignant tumor of urinary system. In China, it also rises to 4th in male and 8th in female. The emergence and development of the bladder carcinoma cause by multifactors steply, and gene mutation may contribute to it. Long - term accumulation of unusual genotypes might result in malignant phenotype. In the past few years, many achievements have been made about genes of bladder cancer. Oncongen activation or Oncongen suppressor gene inactivation develop into can-cerization. Normal cell proliferation results from counterbalance between inhibition genes and promotion genes , such as inactivation of oncongen suppressor genes leads to canceration.Now, many oncongen suppressor genes are clear such as P53, PTCH , PTEN , TSC1etc, and PTEN gene (Phosphatase and tensin homology deleted on chromosome ten, namely and tensin and auxilin isogenesis, No. 10 chromosome lose phosphatase gene) is a newest phosphatas oncongen suppressor regulating cell proliferation , transformation and adhesion etc. By dephosphorylation, PTEN can turn PIP3 into PIP2, and PIP3 is a new dephosphorylation target locating on cell membrane that contributes to cell proliferation and apoptosis. PTEN can inhibit PIP3 dephosphorylate to PIP2. As a result, it restrains signal pathway of mitogen -activated protein kinase(MAPK) and leads to cytostasis or apoptosis. MAPK is a common passage in amniote which impacts cell phenotype and proliferation, and is the common pathway of intracellular messenger transduction to promote cellular proliferatian. It has already verified that PTEN and MAPK are closely related with many kinds of tumour generation. So far there is no re-port on the relation of MAPK and PTEN in bladder tumour.Materials and Methods1. Specimen collections: this study included 70 patients with bladder transitional cell carcinoma from 2002 to 2003 in our hospital.2. Immunohistochemistry: in 70 specimens, PTEN and MAPK protein expression were detected by SP.3. Correlation: analysis correlations between PTEN protein and MAPK in bladder transitional cell carcinoma.Results1. PTEN expression in bladder tumorPTEN were detected in 39% bladder carcinoma, there was not correlation between I type and II type. But no statistically significant correlation was found in T1 stage and T2 stage .2. MAPK expression and locationMAPK was detected in 93% bladder transitional cell carcinoma. The expression of MAPK positively correlated with tumors stage and grade in bladder carcinoma.3. Relationship between the expression of PTEN protein and MAPK .in bladder transitional cell carcinoma.A significant negative relationship was observed between expression intensity of PTEN protein and the phosphorylation level of MAPK in bladder transitional cell carcinoma.Conclusions1. The abnormal expression of PTEN may play an important role in the development of bladder transitional cell carcinoma. PTEN examina - tion might be useful for the judgement of tumor development and prognosis.