Expression Of The Pituitary Tumor-transforming Gene In Endometrial Carcinoma

Objective It has been suggested that pituitary tumor-transforming gene (PTTG),a novel oncogene,has played an important role in many human malignancies. However,its precise role has not been known in carcinogenesis and development of endom carcinoma.In this study,the expressions of PTTG mRNA and protein were detected and the relationship with the clinical-pathological features,the expression of basic fibroblast growth factor (bFGF) protein and microvessel density (MVD) were analyzed in endometrial carcinomas.Methods A total of 56 cases of endometrial carcinomas were examined for the expression of PTTG..PTTG mRNA was detected using semi-quantitive reverse transcription-polymerase chain reaction(RT-PCR),and PTTG,bFGF protein and MVD was detected by immunohistochemistry.The corresponding hyperplasia endometriums and normal endometriums tissues were investigated as control group.Results 1.PTTG mRNA were detected in 85.71% endometrial carcinoma, 40% hyperplasia endometriums and 18.75% normal endometriums tissues.The expression average quantity of PTTG mRNA is 0.8378+0.0752 in endometrial carcinomas,0.6795 0.1238 in hyperplasia endometriums and 0.4973+0.0454 in normal endometriums tissues respectively. The expression rate and average quantity of PTTG mRNA in three groups above demonstrated significant diffrence (P<0.01).2.Among endometrial carcinomas group,higher PTTG mRNA expression quantity significantly correlated with surgical- pathological stage (P<0.01),lymph node metastasis (P<0.01),moymetrial depth(P<0.05) and histological subtype(P<0.05),but it has no association with pathological grade and menstruation status (P>0.05).3.The protein of PTTG was expressed in 62.5% of endometrial carcinomas and 16% of hyperplasia endometriums.but no expression of PTTG protein was found in normal endometriums tissues.The expression of PTTG protein in endometrial carcinomas was significantly higher than in hyperplasia endometriums and normal endometriums tissues4. The expression of PTTG protein was related to the surgical- pathological stage.lymph node metastasis(P<0.01),moymetrial depth(P<0.05) and histological subtype(P<0.05), but it has no association with pathological grade and menstruation status (P >0.05).5.The protein of bFGF was expressed in 55.36% of endometrial carcinomas and 20% of hyperplasia endometriums and 0% in normal endometriums tissues, respectively. The expression of bFGF protein in endometrial carcinomas was significantly higher than in hyperplasia endometriums and normal endometriums tissues (P<0.01).The average quantity of PTTG mRNA in tissues with the bFGF protein coexpression was higher than that in those without bFGF protein coexpression (P<0.05).The expression rate of PTTG protein in tissues with bFGF protein coexpression was higher than that in those without bFGF protein coexpression (P<0.01).6. The MVD was positively correlated with expression of PTTG mRNA (r=0.6486,P<0.01).The MVD in tissue with PTTG protein expression was higher than that in those without PTTG protein expression (P<0.05).Conclusions 1.The expression of PTTG mRNA and protein was higher in endometrial carcinomas than that in hyperplasia endometriums and normal endometriums tissues.The results suggest that PTTG might play an important role in the pathogenesis of endometrial carcinomas.2.Overexpression of both PTTG mRNA and its protein were related to surgical-pathological stage,lymph node metastasis,moymetrial depth and histological subtype in endometrial carcinomas. The results suggest that PTTG may involve in the development and progression of endometrial carcinomas,and PTTG may be as a prognostic marker.3.PTTG together with bFGF may play an important role in carcinogenesis and development of endometrial carcinomas.PTTG induces an angiogenesis,through bFGF,which is a key determinant step in tumor progression and metastatic spread.4.PTTG may be a new target in the treatment of endometrial carcinomas.