A Study Of The Influence Of PSS On Growth Of Cultured Vascular Endothelial Cells And Its Adhesion Function With Polymorphonuclear Leucocytes

Objective: By observing the influence of PSS (Polysaccharide Sulfate, PSS) on growth of cultured vascular endothelial cells (VEC), measuring the level of intercellular adhesion molecule-1 (ICAM-1) released by hypoxia/reoxygenation (H/R)-stimulated VEC(H/R VEC) which were treated with or without PSS, observing the adhesion of normal polymorphonuclear leucocytes (PMN) to H/R VEC which were treated with or without PSS and the change of adhesion function between PMN of cerebral infarction patients and VEC before and after treatment of PSS, in order to investigate the effects of PSS on the adhesion of both VEC and PMN, and therefore to further elaborate the mechanisms of PSS in treating ischemic cerebral disease.Methods: The growth of cultured human umbilical vein endothelial cells-304 (HUVEC-304) were observed by means of MTT which were treated with PSS(final concentration:50,100,150,200,250(ig/ml) or without PSS separately. Hypoxia/ reoxygenation (H/R) model of VEC was established, the VEC were treated with PSS(50,100,150,200,250|ig/ml) or without PSS ,the level of soluble ICAM-1 were measured and the adhesion of polymorphonuclear leucocytes (PMN) from normal people to VEC were observed in each group, and then the best final concentration of PSS in decreasing the adhesion function of VEC were selected. 60 cases of cerebral infarction were divided randomly into two groups: PSS group(treated with regular medicine plus PSS) and non-PSS group(treated with regular medicine only). The adhesion of PMN from cerebral infarction patients to cultured VEC were determined before and after treatment of PSS.Results: The growth of VEC in PSS-treated (50,100,150,200ng/ml) groups were much better than that of non-PSS-treated group, especially in 100μg/ml PSS final concentration group. Compared with the control, the adhesion of cultured VEC to normal PMN were decreased significantly in PSS-treated (100,150,200μg/ml) groups, and the level of soluble ICAM-1 in those groups were also decreased significantly, especially in 150μg/ml PSS final concentration group. The adhesion of PMN from patients with acute...