| Hyperlipidemia is defined as one or several lipids are higher than normal levels in plasma because the metabolism and transport of fats are abnormal. Dyslipidemia is nearly associated with cardiovascular diseases, which is one of the primary dangerous factors to atherosclerosis and coronary heart disease. Based on the elevation of various plasma lipoprotein, hyperlipidemia is classified into six types in clinic: Typeâ… , plasma TG levels are very high, but TC concentrations are normal or little increase; typeâ…¡a, plasma TC is elevated while TG may be normal; Typeâ…¡b, both TC and TG are high; Type â…¢, both TC and TG levels are very high; Type â…£, TG levels are significantly elevated and TC levels are normal or little elevation; Typeâ…¤, both TG and TC are increased, but the increase of TG is primary. At present, the animal models of hyperlipidemia and atherosclerosis are induced by means of breeding, injection, stress, and immunity. In Europe and America, the transgenic animal model of hyperlipidemia has been developed using molecular biology and gene engineering technology. However, there are some disadvantages in these animal models, for example the period is longer, the process is more complicated, and the TG concentrations can not be raised obviously. The aim of the present work is to establish a fast and easy hyperlipidemic animal model by the administration of bifendate pill, an analog of schisandrin C. This model can be used for studying the blood lipid-lowering drugs and the lipid metabolism. 1. Effects of various vehicles on bifendate pill-induced hypertriglyceridemia Bifendate pill (0.1 g/kg,0.3 g/kg,1 g/kg) was suspended in 0.5% CMC, olive oil, or salad oil. When suspended in 0.5% CMC and given to mice orally, after 24 h bifendate pill increased the TG levels for 27% at 0.1g/kg, 29% at 0.3g/kg, and 71% at 1 g/kg (P<0.01) compared with control. When mice were intragastrically administrated with bifendate pill (mixed with olive oil) at doses of 0.1 g/kg,0.3 g/kg,1 g/kg, after 24 h the TG contents in serum were elevated by 48%, 72% and 71%, respecticvely (P<0.01). When suspended in salad oil, bifendate pill increased the serum TG for 46% (0.3 g/kg) and 54% (1 g/kg) (P<0.01). 2. Effects of bifendate pill on blood and liver tissue lipids in normal animals 2.1 Effects of bifendate pill on blood and liver TG and TC in mice: Mice were orally treated with bifendate pill suspended in 0.5% CMC for once and once daily for four days. Results showed that bifendate pill at dose of 1 g/kg elevated liver TG concentrations for 31% (P<0.05) at 6 h after drug administration. Bifendate pill at doses of 0.25, 0.5 and 1 g/kg increased serum TG for 39%, 46% and 76% (P<0.01 or P<0.05) and liver TG for 33%, 23% and 17% (P<0.05) at 24 h intervals after drug administration. Bifendate (1 g/kg) can also elevate serum TG for 39% at 48 h intervals (P<0.01). At the same time, bifendate pill 0.1 g/kg increased liver TG for 18% (P<0.01) and decreased liver TC for 9% (P<0.05). Mice were treated with bifendate pill at 0.25 g/kg and 1 g/kg for successive four times. Bifendate pill at 1 g/kg increased serum TG levels 30% at 6 h intervals after the last dosing (P<0.05). Bifendate pill at the doses of 0.25 and 1 g/kg increased serum TG levels 56% and 79% (P<0.01) at 24 h and 24% and 25% (P<0.05) at 48 h after the last dosing, respectively. Bifendate pill 0.25 g/kg can increase liver TG levels 34% (P<0.01) at 24 h and 30% (P<0.05) at 48 h after the last dosing. 2.2 Effects of bifendate pill on serum TG and TC in rats: Rats were intragastrically treated with bifendate pill at the doses of 0.03, 0.1, 0.3 and 1 g/kg once daily for 8 days. Results showed that bifendate pill did not influence the serum TG and TC levels in rats at 2, 5, and 9 days after drug adiministration. 2.3 Effects of bifendate pill on serum TG and TC in rabbits: Rabbits were orally administered with bifendate pill at the dose of 0.3 g/kg suspended in 0.5% CMC. After 3 h, 6 h, 12 h, 18 h, 24 h, 36 h, 48 h and 72 h the blood samples were obtained from ear vein. Then serum T...
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