A Study Of Association Between The Polymorphism Of Endothelial Nitric Oxide Synthase (eNOS) Gene 27bp Variable Number Of Tandem Repeats (VNTR) And Restenosis After Coronary Stenting

Percutaneous coronary interveration (PCI) has a consequence in curing coronary heart disease.however restenosis has been restricted its applications.Recently,srudies have show that blood vessel inner film hyperplasia overage play an important role in mechanism of RS.Few reports about this process related gene type of individual. Recently srudies have show the associations between NOS polymorphism and coronary heart disease(CHD).Polymerase chain reaction restricted fragment length polymorphism was used to test 105 patients gene type with coronary stenting.To investigate the association between nitric oxide synthase (NOS) polymorphism and RS by examine patients, genes.To explore high dangerous RS patients and direct individual therapy.Objective To investigate the association of the polymorphism of endothelialnitric oxide synthase (eNOS) gene 27bp variable number of tandem repeats (VNTR) with restenosis after coronary stenting in Chinese population. Methods (1) One hundred and five cases were enrolled who underwent coronary stenting and coronary angiography at least 3 months after the procedure. The information of clinical risk factors and procedure-related factors was collected; (2) Genotypes of 105 patients were determined by polymerase chain reaction (PCR)and agarose gel electrophoresis and gene sequenciong.(3) Fasting serum nitric oxide metabolite (NOx) and Endothelin (ET) were measured by nitrate reductase and radioim munoassay respectively. Results (1) In total 105 enrolled patients, 65 cases developed in-stent restenosis, and the others were free from restenosis.(2) Two alleles, containing4and5, and three genotypes namely 4/4-homozygous, 4/5-heterozygous, 5/5-heterozygous, were indentified in both the patients with restenosis and the patients without restenosis. The overall distributions ot either allele or genotype frequencies differed significantly between the two groups, the genotypes of the 4 repeats allel, 4/5 -heterozygous and 4/4-homozyguswere more frequent in the patients with restenosis group than without restenosis group. Conclusion The 4 repeats allele in eNOS gene might have a higher risk of restenosis after coronary stenting. The 4 repeats allele in eNOS gene may be induced the procedure of restenosis by reducing NO release of endothelium and damaging the...