Application And Mechanism Of Chiral Pharmaceutical Analysis By Capillary Electrophoresis

Bioactivity of chiral drugs is closely related to the stereo structures. Nowadays the production and usage of optically pure drugs is the trend of new drugs research. The study on chiral drugs has become one of new directions of new drugs research internationally. Analysis of chiral drugs, with increasing importance in pharmaceutical research, is now a hot topic in international analysis science.At present analysis of chiral drugs is mainly achieved by two approaches: direct and indirect enantioseparation. Direct enantioseparation is simple, rapid, accurate and reliable. High performance liquid chromatography (HPLC) plays a leads role in direct enantiseparation, while the applications of capillary electrophoresis (CE) are increasing significantly. CE, developed rapidly in last two decades, is a fast separation technique with high efficiency and sensitivity.In CE enantioseparation, the chiral selector is dissolved in the running buffer to provide chiral environment. So the two enantiomers can be separated owing to the difference of the interactions between chiral selectors and enantiomers and thus the difference of electrophoretic motilities. Cyclodextrins (CDs) are the most widely used chiral selectors. Presently CE enantioseparation is focused on experimental research between different kinds of cyclodextrins and enantiomers, while lacking of mechanism research systematically.Application research on analysis of isomer drugs by CE was carried out at the same time by the angles and demands of pharmaceutical analysis, such as (1) Research on impurity test of enantiomer in ephedrine levorotatory using CE in cyclodextrin two-dimension system; (2) Research of appliying CE to test Pramipexole dextrorotatory in Pramipexole levorotatory; (3) Study on CE separtion of two pair enantiomers-SIPIyy24A and SIPIyy24B using difluorophenyl carboxymethyl- β -CD. (4) Capillary electrophoresis for chiral separation of six β -blocking agent.In this report, the computer was used to simulate the classic theory "three-point interaction" for chrial separation, which furthermore was quantized by applying...