Expression And Significance Of STAT3 And Related Genes In Seminma

Objective: By studying the expression of Stat3 in seminoma, to confirm wether Stat3 play a role in the occurring of seminoma by the effection of induce and impulse;By the research on VEGF and C-myc ,understand the hyperplasy caused by the JAK/STAT3 signal transduction pathway in this tumor;By the investigation in HIWI and Bcl-xl, confirm the anti-apoptosis effection caused by STAT3/Bcl-xl in the development of seminoma,so,the aim of this topic is to provide an necessary theory basement for the clinic therapy in seminoma ,which is to aim directly at STAT3.Methods: 38 paraffin specimen of seminoma and 10 paraffin specimen of nomal human testis were collected.IHC were used to detect the protein of Stat3,VEGF,C-myc and Bcl-xl; ISH were used to observe the mRNA transcription of HIWI .Results:1.The positive expression rate of STAT3,VEGF,C-myc and Bcl-xl is 76.3%,71.1%,84.2%,71.1%,and comparing nomal testis they all had different significance in statistics(p<0.01),and these genes all have relationship with the TNM clinical stage.2.The positive expression rate of HIWImRNA was 68.4%,comparing nomal testis it had different significance in statistics(p<0.05).But it was none of the business of the TNM clinical stage.3.There were correlation among STAT3,VEGF,C-myc,Bcl-xl and HIWI genes.There was positive correlation between STAT3 and VEGF(r=0.640,p<0.01), STAT3 and C-myc(r=0.408,p<0.05),C-myc and VEGF(r=0.459,p<0.01),HIWI and STAT3(r=0.679,p<0.01),HIWI and BCL-xl(r=0.602,p<0.01),STAT3 and Bcl-xl(r=0.669,p<0.01). Conclusions:1.The JAK/STAT3 signal transducer pathway could activate the expression of VEGF,and impulse the development of seminoma.2.The JAK/STAT3 signal transducer pathway could increase the expression of C-myc and cause the malignancy hyperplasy of germinal cell,which provocating the seminoma.3.In seminoma,the gene HIWI could activation the STAT3/Bcl-xl signal transducer pathway,give tumor cell the ability of self-renewal and anti-apoptosis.