Effect Of Intra-articular Sodium Hyaluronate Injection For Chondrocyte Apoptosis Following Acute Osteochondral Injury

There are two parts in our study. Part 1: Experimental study on chondrocyte apoptosis following acute osteochondral injury ; Part 2: Experimental study on effect of intra-articular sodium hyaluronate injection for Chondrocyte apoptosis following acute osteochondral injury. Abstract of each part described below: Part 1: Experimental study on chondrocyte apoptosis following acute osteochondral injury Objective To explore the characteristics of chondrocyte apoptosis following acute osteochondral injury. Methods Drill holes were created in rabbit knees.TUNEL, transmission election microscopy(TEM), fluorescence-activated cell sorter(FACS) were applied to study the injured femoral condyles obtained at different intervals following drill injury. Results On post-injury day 4, in injured cartilage specimens displayed a statistically significant increased overall level of apoptosis, most of apoptotic chondrocytes were confined to the superficial tangential zone and the middle zone. Conclusions Chondrocyte apoptosis may contribute to the subsequent development of post-traumatic arthritis. It would be important to prevent the knee from post-traumatic arthritis by repressing chondrocyte apoptosis as early as possible. Part 2: Experimental study on effect of intra-articular sodium hyaluronate injection for chondrocyte apoptosis following acute osteochondral injury Objective To explore the effect of intra-articular sodium hyaluronate injection for Chondrocyte apoptosis following acute osteochondral injury. Effect of Intra-articular Sodium Hyaluronate Injection for Chondrocyte Apoptosis Following Acute Osteochondral Injury Abstract Methods Adult New Zealand White rabbits underwent 2 ㎜osteochondral drilling of the medial and lateral femoral condyles of both hind limbs. Then choosed one knee randomly as experimental side, the other as control side. The knee in experimental group was injected with 1% sodium hyaluronate at the end of the operation and then every week postoperatively.The control group was not treated by any injection.TUNEL, fluorescence-activated cell sorter(FACS) were applied to study the injured femoral condyles obtained at different intervals following drill injury. Results Chondrocyte apoptosis in the experiment group was significant reduce in the experiment group compared with control group. Conclusion Intra-articular sodium hyaluronate injection just after acute osteochondral injury can suppress chondrocyte apoptosis , it would be very important to prevent the knee from post-traumatic arthritis.