Clinical Significance Of Expression Of PTEN And Survivin In Laryngeal Squamous Cell Carcinoma And Lymph Node Metastases

Objective:1 To investigate the clinical significance and the correlation of tumor suppressor gene PTEN and inhibitor of apoptosis gene survivin in laryngeal squamous cell carcinoma (LSCC) and to afford some information about the early diagnosis, progression and therapy of LSCC.2 To investigate the difference of the primary foci and the metastases foci in LSCC at molecular lever.Methods:S-P immunohistochemistry1 Examined the expression of PTEN and survivin in 57 cases of LSCC, 27 cases of adjacent safety margin (ASM) randomized drawn from the LSCC and 22 cases of vocal cord polyp (VCP).2 Evaluated the expression of PTEN and survivin between the primary foci and the metastases foci in the 18 cases with lymph node metastases among the 57 cases of LSCC.Statistic analyses were done by SPSS 12. 0 software. Results:1 The positive rates of PTEN in LSCC, ASM and VCP were respectively 89. 5 % (51/57), 88.9% (24/27) and 95.5% (21/22), there were no significant difference among them (P >0.05). But the expression degrees in both LSCC and ASM were weaker than in VCP (P <0. 01), the expression degrees in LSCC and ASM had no significance (P>0. 05).2 There were no expression of survivin in VCP tissues, the positive rates of survivin in LSCC and ASM were respectively 50. 9% (29/57) and 11. 1% (3/27). The expression rates in LSCC and ASM, LSCC and VCP were significant (P <0. 01), but the expression rates between ASM and VCP had no significance(P>0.05) . The expression degrees of LSCC and ASM had no significantdifference (P >0.05).3 Expression of PTEN or survivin had no significant difference among different tumor site, differentiation, T classification, clinical stage, nodal metastases and etc (P >0. 05).4 There were no correlation between PTEN and survivin expression in LSCC(r=-0. 15, P>0. 05).5 There were no expression either PTEN or survivin in negative lymph nodes.The positive rates of PTEN in LSCC primary foci and lymph node metastases foci were the same (94. 4%), but the expression degrees were more powerful in the metastases foci than in the primary foci (P <0. 05). The positive rates of survivin in LSCC primary foci and lymph node metastases foci were respectively 55.5% (10/18) and 50.0% (9/18), neither the positive rates nor the expression degrees had significant difference between the both tissues (P >0. 05). Conclusions:1. During the carcinogenesis of LSCC, partial mutation of PTEN may occured, this change was the early molecular event of the carcinogenesis.2. Survivin probably played an important role during the carcinogenesis of LSCC, this change was the early molecular event of the carcinogenesis.3. Talk about PTEN or survivin, there was no relationship to the biological behavior in LSCC, such as progression, metastases and etc.4. There was no correlation in LSCC between PTEN and survivin expression.5. There was difference between the LSCC primary foci and the lymph node metastases foci in molecular level probably.