| Objective To evaluate the effects of interleukin 10 on pancreatic ischemia of experimental acute necrotizing pancreatitis(ANP). Methods 92 Spraque-Dawley rats were randomly divided into control group(group C, n=24), ANP group(group A, n=36) and IL-10 group(group I, n=32). ANP of rats were induced by three times intraperitoneal injection of 6% L-Arginine (3×1.0mg/g) for hourly interval. Group I was treated with 10,000 unites of recombination human IL-10(rhIL-10) by intraperitioneal injection at 2th, 5th and 8th hour after the last L-arginine injection. Rats were killed at the 4th, 12th, 24th and 36th hour after the last L-Arginine injection. Serum levels of amylase, thromboxane B2(TXB2) , 6-keto-PGF1α were assayed. Results Compare with group C, Serum level of amylase,TXB2 and 6-keto-PGF1α of group A was significantly(P<0.01) higher at different time point. After treated with rhIL-10, serum levels of amylase, TXB2 was significantly decreased(P<0.05 or P<0.01). Conversely, serum 6-keto-PGF1α was increased (P<0.01). The TXB2/6-keto-PGF1α ratio was markedly diminished too(P<0.01). Conclusion Microcirculatory disturbance is an important impairment factor in acute pancreatitis. IL-10 can improve the pancreatic ischemia in ANP rats by keeping the balance of TXB2/6-keto-PGF1α ratio through cytokine pathway and may be an effective therapy for acute pancreatitis treatment.
|
PDF Full Text Request