Expression Of Cyclin E And P53 In Oral Squamous Cell Carcinoma

Purpose:OSCC is the most type in oral-maxillofacial Benign tumors which accounted for 80% and threatens the patients's lives. Like other tumors,the occurrence of OSCC is induce by deregulation of cell cycle resulting in the abnormal prolifertion and differentiation of cells. So far there is no clear understanding on the etiology and pathogenesis of this neoplasm.To study onthew cell cycle regulation and molecular mechanism would benefit to elucidate the tumorrigenesis of osteosarcoma and establish a new method for treatment. The running of cell cycle is regulated by cycling CDK and CDKI.cyclin-CKD is a positive regulative factor of cell cycle,which can promote the progress of cell cycle . CDKI is a negative regulative factor of cell cycle,and it can block progression of cell cycle,inhibit over-proliferetion of cells. Cyclin E is one of positive regulation factors in G1 period.It combines CDK and promotes the DNA synthesis to realize the switch from Gl to S period. It has been confirmed that Cyclin E is a oncogene which gene amplificate and overexpress in many tumors. P53 regulates the cell cycle through indirectly inhibiting G1/S change-over and has some relationship with Cyclin E. The disturbance of the cell cycle has relationship with neoplasms,such as gastric carcinoma, pulmonary carcinoma, conolic cancer anf so on . There is no report on the expression of them and the correlation between them in OSCC in our country.In our experiment ,we used immunohistochemistry to study the expression of Cyclin E and P53 in development of OSCC and explore the relationship of clinicopathologic significance.Objectives of this research include:1. Examined expression of Cyclin E,P53 protein in normal mucosa, leukoplakia,squamous cell carcinoma in oral2. Investigating expression of Cyclin E,P53's relationship with pathological type,metastasis,TNM in OSCC3. 3. Investigating expression of Cyclin E's relationship with expression of P53 in OSCC.Materials and Methods1.38 leukoplakias(10 non-atypical hyperplasia;28 atypical hyperplasia) and 52 oralsquamous cell carcinomas were selected.Information of these cases was collectedincluding age,gender,location,pathological type,metastasis and TNM stage. 10 normallingual mucosas were used as controls.2.Using immuohistochemical SP staining,we examined Cyclin E and P53 proteinexpression in paraffin slices of 38 leukoplakias and 52 oral squamous cellcarcinomas. As controls,expression of these proteins in 10 normal oral mucosas werealso detected.3.Difference of Cyclin E and P53 were analyzed in normal mucosas, leukoplakias andoral squamous cell carcinomas,Relationships of these protein expression withpathological type,metastasis and TNM stage were also analyzed in OSCC,and so wererelationship of Cyclin E expression with P53 expression.RESULT1 Cyclin E was negative expression in normal mucosa or leukoplakia(non-atypical hyperplasia)and positive rate was46.4% (13/28) in leukoplakia(atypical hyperplasia) and 65.4% (34/52) in OSCC.the Cyclin E positive rate in Gland G2,G3 group was 58.3% .. 66.7% > 69.2%.There was no statistical difference Gl and G2,G3 group(P>0.05)and between( I + II )and (III+IV) group (P>0.05),and was statistical diference between LN- and LN+ group (P<0.05) .