| Objective Soybean isoflavone(SI) has been a component of the diet of certain populations for centuries including three isoflavones aglycones with the common names genistein, daizdain, and glycitein. In recent years, the research on its biological activities has been reported frequently. SI has been shown to regulate bodies' endocrine function, inhibit the proliferation of breast cancer, gastic adenocarcinoma and prostate cancer, etc in vivo and in vitro, especially, prevent tumor, treat tumor and postpone cancer . In this article, we have only observed the effect of SI on the frequency of sister chromatid exchange in lymphocyte from human peripheral blood, micronuleus formation of binuclear cell and frequency of hgprt genetic mutation and the protective effect on DNA damage in CHL cells in vitro, but also we observed SI's actions of antitumor.Methods 1 The effect of SI on lymphocytes and CHL cells in genetic damage and the protective effect on genetic damage. (l)The different doses of SI are 0.5,5,50μmol/L respectively. (2)SCE test : we observed the effect of SI/SI+MMC on the frequencies of SCE in lymphocytes from human peripheral blood in SCE test. (3)CB-MNT and hgprt gene mutation test: the effect of SI/SI+MMC on the frequencies of micronuleus and hgprt gene mutation in CHL cells was observed. (4)SCGE: we obeserved the effect of SI/SI+PB on DNA damage of CHL cells. 2 The inhibiting effect SI on human breast cancer cells MCF-7. (l)The different doses of SI are 10,30,100μmol/L respectively. (2) The effect of SI on MCF-7 in proliferation : after cultured with SI from 1 to 8 days, the OD of MCF-7 were measured . (3) The effect of SI on MCF-7 in cells mitotic index : after cultured with SI 1 to 8 respectively, dealed with Giemsa , the mitotic index were measured bymicroscopy . (4) The effect of SI on MCF-7 in cell apoptosis index : after cultured with SI 48h and 96h, observed the apoptosis index. (5) The effect of SI on MCF-7 in cell anchoring rate. (6) The primary discussion of SI on antitumor's pathway: Set two groups, using E2 (10"9mol/L) as positive control, one group with the end concentration 0.01, 0.1, 1, 10, 20, 40, 80, lOOumol/L respectively, the other group for MTT with E2 added, then the effects on MCF-7 cells and MDA-MB-231 cells are observed by SI only and the union of SI and E2.Results 1 The effct of SI on lymphocytes and CHL cells in genetic damage and the protective effect on genetic damage: (1) The frequency of sister chromatid exchange(SCE) of lymphocytes, the frequency of micronuleus in double-nucleus cells ,the frequency of hgprt gene mutation and the effect of DNA damage on CHL cells compared with negative control exhibited no statistical significance (P>0.05). (2)The SCE of lymphocytes treated with SI plus MMC compared with positive control(MMC) were markedly decreased(P<0.05), and such decreases were dose related(r/= -0.618, .P=0.001). (3)The frequency of micronucleus treated with SI plus MMC compared with positive control (MMC) were decreased in dose of 0.5,5,50umol/L (PO.05), and such decreases were dose related(r,= -0.719, P=0.000); but the frequency of hgprt gene mutation compared with positive control exhibited no statistical significance(P>0.05). (4)The protective effect SI on DNA damage were only observed in dose of 50umol/L treated with SI plus PB compared with positive control(PB) (P<0.05). 2.The inhibiting effect of SI on human breast cancer cells MCF-7: (l)Cell inhibitory concentration of 30% (IC30) and 50% (IC50) are 17.5 and 32.0ujtnol/L respectively. (2)Cell mitotic index : When cultured for 1 to 8 days, the mitotic index was increased first ,then decreased. We can observe the peak in each concentration . (3)The apoptosis index: The significant difference of SI between 10, 30 and lOGumol/L group in 48h and 96h, that is, the apoptosis index of in 10,30 and lOOumol/L group was higher than control group in the same time(.P<0.05), but in the different time we have not observed the difference on the same doses. (4)Cell anchoring rate: SI can decrease the cell anchoring rate of...
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