The Expression Of PD-ECGF, BFGF In Colorectal Cancer And Their Relationship With Microvessel Density(MVD)

[Objective]To study the expression of PD-ECGF and bFGF in colorectal cancer and their relationship with tumor angiogenesis, clinicopathologic feature and prognosis. [Methods]Immunohistochemical technique was used to detect the expression of PD-ECGF and bFGF in 80 cases of colorectal cancer, the microvessel density(MVD) marked by CD34 was also detected. The relationship between the expression of PD-ECGF, bFGF and tumor angiogenesis, clinicopathologic feature, prognosis of patients was then analyzed. 30 cases of colorectal adenoma and 30 cases of normal colorectal tissue were selected as contrast group. [Results]The MVD of colorectal cancer(30.2±6.3) was significantly higher than that of colorectal adenoma(13.1 ±1.6)and normal colorectal tissue(6.5 ±2.7), P<0.01. The MVD of colorectal cancer was significantly correlated with Dukes stage, invasive depth and lymph-node metastasis, P<0.01.In the cases of colorectal cancer ,the 5 years survival rate from the Kaplan-Meier survival analysis in the high MVD group(35.71%) was significantly lower than that in the low MVD group(71.05%), Logrank=12.11, P=0.0005.The positive expression rate of PD-ECGF in colorectal cancers(71.3%) was remarkably higher than that in colorectal adenomas (43.3%) and normal colorectal tissues (10.0%) , P<0.01. In colorectal cancer, the expression of PD-ECGF was remarkably associated with Dukes stage, differentiated degree, invasive depth and lymph-node metastasis, P<0.05. The MVD of the PD-ECGF positive expression group (33.6 ± 6.6) was remarkably higher than that of the negative expression group (26.3 ± 2.2 ), P=0.009; the positive expression rate of PD-ECGF in high MVD group(88.1%) was remarkably higher than that in low MVD group(52.6%), P=0.001. In the cases of colorectal cancer, the 5 years survival rate from the Kaplan-Meier survival analysis in the PD-ECGF positive expression group(43.86%) was remarkably lower than that in the negative expression group (73.91%), Logrank=13.38, P=0.0003.The positive expression rate of bFGF in colorectal cancers(57.5%) was significantly higher than that in colorectal adenomas (30.0%) and normal colorectal tissues (6.7%) , P<0.05. In colorectal cancer, the expression of bFGF was significantly correlated with Dukes stage, invasive depth and lymph-node metastasis, P<0.05. The MVD of the bFGF positive expression group (32.2 + 3.7) was significantly higher than that of the negative expression group (28.3 ±3.5 ), P=0.030; the positive expression rate of bFGF in high MVD group(73.8%) was significantly higher than that in low MVD group(39.5%), P=0.003. In the cases of colorectal cancer ,the 5 years survival rate from the Kaplan-Meier survival analysis in the bFGF positive expression group(43.48%) was significantly lower than that in the negative expression group (64.71%), Logrank=8.15, P=0.0043.In the cases of colorectal cancer, the MVD of both PD-ECGF and bFGF positively expressed group, one of them positively expressed group, both of them negatively expressed group were 34.5±5.8, 29.2±2.9, 24.6±7.7 respectively, P<0.05; the incidence rates of lymph-node metastasis in the above-mentioned three groups were 71.1%, 44.4%, 13.3% respectively, P<0.05. There was a positive correlation between the expression of PD-ECGF and bFGF, r=0.292, P=0.009. [Conclusion]1. The overexpression of PD-ECGF and bFGF in colorectal cancers have a significant relation with tumor angiogenesis.2. PD-ECGF, bFGF and MVD are significantly correlated with Dukes stage, invasive depth and lymph-node metastasis of colorectal cancer, and PD-ECGF is correlated with differentiated degree; their overexpression may lead to advanced invasion of colorectal cancer and unfavorable prognosis.3. PD-ECGF and bFGF may have play a corporative action in colorectal cancer, their simultaneous expression may lead to tumor angiogenesis increasing greatly, cancer invading and metastasizing easily.4. The combinative detection of PD-ECGF and bFGF in colorectal cancer have the positive significance of the judgement of malignant phenotype and prognosis, and guide to the clinical treatment.