The Research Of Expression Of MMP-9,p21～(ras) And BFGF In Colorectal Neoplasma And Their Clinical Values

Invasion and metastasis are important biologic actions of malignant tumors. They are also leading causes of cancer death. Metastasis causes cancer cells to implant widely and heterogeneous cancer cell ingredients, which makes present therapies (including operation, chemotherapy, radiotherapy) inefficient. Colorectal carcinoma is one of the common malignant tumor, and its incidence rate and death rate both rank the forefront of all the malignant tumors. As other tumors, colorectal carcinoma also has these characteristics such as invasion and metastasis in processes of its occurrence and development, but the mechanism has not been cleared. Studies showed that proteolytics are closely related to malignant tumor's invasion and metastasis. Among these proteolytics, matrix metalloproteinases' action was especially striking. MMPs degrade cells basement membrane and extracellular matrixes, thus promoteinvasion and entry into and out of blood and lymphatic vessels and formation of metastases at distant sites. Being a number of MMPs family, MMP-9 plays a vital role in tumor cells' invasion and metastasis. Through degrading collagen type IV V VII X and gelatin FN, MMP-9 realizes its above mentioned actions. p21ras stimulates MMP-9 mRNA ignitor to some extent, thus regulates the expression of MMP-9 protein. MMP-9 degrades epithelial basement membrane, which makes blood vessel growth factor- basic fibroblast growth factor (bFGF) release from these cells. bFGF is involved in the formation of tumor blood vessel endothelial at both primary and distant sites, thus keeps solid tumors existing and developing. In order to investigate the mechanism of invasion and metastasis and thus provide theory basis of preventing and treating malignant tumor centring on MMPs, an immunohistochemical Streptavidin-peroxidase (SP) method was used to examine the expression of MMP-9 p21ras and bFGF in colorectal carcinoma and the correlation between MMP-9 and p21? MMP-9 and bFGF.Materials and methods: (1) 66 surgically resected colorectal adenocarcinoma rectum samples and 3 1 adenoma samples and 21 normal mucosa samples of the colon and rectum which were adjacent to adenocarcinoma mucosa but were confirmed pathologically as normal mucosa. All the tissues were fixed in 10% neutral formalin and embedden in paraffin. (2) SPimmunohistochemical staining technique was used to examine the expression of MMP-9 p21ras and bFGF in normal mucosa, adenoma and colorectal adenocarcinoma. (3) The data were analyzed by software SPSS 10.0, x 2-test was used to compare differences between groups and Spearman grade correlation test was used to analyze the correlation between MM-9 and p21ras MMP-9 and bFGF. Statistically significant level was considered as "alpha equals 0.05" and the correlation was considered positive if the value was above 0.Results:1. In normal mucosa, positive staining for MMP-9 was seen in scattering few epithelial cell and the positive rate was 0%; positive staining for MMP-9 in colorectal adenoma was even, and the positive cells were found at the top of the mucosal layer, and the positive rate was 61.29%; in the colorectal adenocarcinoma, positive staining for MMP-9 was not even and its positive rate was 78.79%. There were significant differences (PO.01) between normal mucosa group and adenocarcinoma group but no between adenoma group and adenocarcinoma group (P>0.05). The positive rate for p21ras in normal mucosa was 0%; positive staining for p21ras in colorectal adenoma was thin and even, and the positive cells were found most at the top of the mucosal layer and the positive rate was 25.81%; positive staining for p21ras in the colorectal adenocarcinoma was not even and the positive rate was 46.97%. There were significant differences (PO.05)between normal mucosa group and adenocarcinoma group but no between normal mucosa group and adenoma group, adenoma group and adenomacarcinoma group (P>0.05); the positive rates of bFGF in normal mucosa, adenoma and adenocarcinoma were 4.76%, 58.06% and 60.00% respectively. Whe...