Effect Of Radio-frequency Ablation On Microvessel Density And Vascular Endothelial Growth Factor In Rabbit Hepatic Carcinoma Models

Objective: As a new techque of local therapy for liver cancer, radio-frequency ablation (RFA) was widely used in recent years, but the possible mechanisms of RFA have not been clarified. This study was to observe the histopathological change of periphery hepatic cancer tissue around RFA on microvessel density (MVD) and vascular endothelial growth factor (VEGF) in the rabbit hepatic cancer models.Methods: The VX₂ tumor cells were planted in the hepatic left-central lobe on 32 rabbits. All animals were divided to 8 groups randomly (4 each groups), the control group (1 group, n=4) and the RFA groups (7 groups, n=28). The expression of MVD and VEGF in the periphery hepatic cancer tissue around RFA were observed by immunohistochemical SP method and measure the control group and 0 2 6 12 24, 48 168h after the operation on RFA groups.Results: The expression of MVD and VEGF in the periphery hepatic cancer tissue after RFA treatment in the RFA groups, which were decreased significantly compared with those in the control group (P<0.01), However, expression of MVD and VEGF in the tumor tissue after RFA treatment in the RFA groups do not differ from each other (P>0.05).Conclusions: RFA treatment can effectively destroy the micovessel of the periphery hepatic cancer tissue around RFA, and repress the vascularization and reduce the blood supply of hepatic carcinoma.