Effects Of Celecoxib On Human Osteosarcoma Cell Line--HOS-8603

Obiective: To evaluate the effects and mechanisms of action of celecoxib in inhibiting proliferation and inducing apoptosis on human osteosarcoma cells.Methods: The proliferation of human osteosarcoma cell line--HOS-8603 was measured by MTT assay .The level of prostaglandin E₂ secreted by osteosarcoma cell was detected by High Performance Liquid Chromatogram assay. The expression of P21WAF1 mRNA were detected by semi-quantitative RT-PCR . The content of Cyclooxygenase-2 was shown by Western blot assay. Apoptosis was measured using FACS.Result: The inhibitory rate of celecoxib on HOS-8603 cell was46.75 ± 7.697 %, if HOS-8603 cell has been treated with 80umol/L celecoxib for 48 hours. By FACS, it was found that the rate of apoptosis cells were increased up to 46.0%(control group was 1.3%). The result of Western blot assay revealed that apoptosis rates of different concentrations of celecoxib accorded with the content of Cyclooxygenase-2. The result of RT-PCR shows that P21WAF1 gene expression has been up-regulated by celecoxib. But these effects of celecoxib on HOS-8603 cells can't be counteracted by prostaglandin E₂:When we added 1.5mg/L prostaglandin E₂ to cells that were treated with 80umol/L celecoxib,the inhibitory rate and apoptosis rate were 46.30 ± 6.812% , 45.8%(control group was 1.3%).Conclusion: Celecoxib played an inhibitory effect and induced apoptosis on HOS-8603 cells.And the probable mechanism had something to do with the up-regulation of P21WAF1 gene expression and the up-regulation of COX-2. But these effects of celecoxib can't be counteracted by prostaglandin E₂.